Donkey Anti-Goat IgG H&L (Cy2 ®) preadsorbed (ab6948)
Key features and details
- Donkey Anti-Goat IgG H&L (Cy2 ®) preadsorbed
- Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
- Host species: Donkey
- Suitable for: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA
Related conjugates and formulations
製品の概要
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製品名
Donkey Anti-Goat IgG H&L (Cy2 ®) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Goat -
アプリケーション
適用あり: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details -
吸着処理血清
Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat more details -
免疫原
Full length native Goat IgG (purified).
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標識
Cy2 ®. Ex: 489nm, Em: 505nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. -
特記事項(標識)
Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 3.9 moles Cy2 per mole of Donkey IgG -
ポリ/モノ
ポリクローナル -
特記事項
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6948の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/1000 - 1/5000.
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IHC-P |
Use at an assay dependent concentration.
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|
IHC-Fr |
Use at an assay dependent concentration.
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|
ICC |
Use at an assay dependent concentration.
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|
Flow Cyt |
Use at an assay dependent dilution.
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|
ELISA |
1/10000 - 1/50000.
|
特記事項 |
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ICC/IF
1/1000 - 1/5000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
ELISA
1/10000 - 1/50000. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab6948 は 2 報の論文で使用されています。
- Amo L et al. Podocalyxin Expressed in Antigen Presenting Cells Promotes Interaction With T Cells and Alters Centrosome Translocation to the Contact Site. Front Immunol 13:835527 (2022). PubMed: 35711462
- Barsi JC et al. Mind bomb1 is a ubiquitin ligase essential for mouse embryonic development and Notch signaling. Mech Dev 122:1106-17 (2005). PubMed: 16061358