Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
Key features and details
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed
- Conjugation: Alexa Fluor® 750. Ex: 749nm, Em: 775nm
- Host species: Donkey
- Suitable for: IHC-Fr, ELISA, ICC/IF, Flow Cyt, IHC-P
製品の概要
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製品名
Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed
IgG 二次抗体 製品一覧 -
由来種
Donkey -
ターゲット生物種
Goat -
特異性
By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species. -
アプリケーション
適用あり: IHC-Fr, ELISA, ICC/IF, Flow Cyt, IHC-Pmore details -
吸着処理血清
Chicken, Human, Mouse, Pig, Rabbit, Rat more details -
免疫原
Full length protein corresponding to Goat IgG.
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標識
Alexa Fluor® 750. Ex: 749nm, Em: 775nm
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol, PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. -
ポリ/モノ
ポリクローナル -
特記事項
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab175745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. This applicaiton is no longer batch tested |
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| ELISA | Use at an assay dependent concentration. This applicaiton is no longer batch tested |
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| ICC/IF | 1/200 - 1/1000. This applicaiton is no longer batch tested |
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| Flow Cyt | 1/2000. This applicaiton is no longer batch tested |
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| IHC-P | Use at an assay dependent concentration. This applicaiton is no longer batch tested |
画像
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Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)Anti-GAPDH antibody - Loading Control (ab9483) at 1 µg/ml + HeLa Whole Cell Lysate at 10 µg
Secondary
Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745) at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 37 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with ab9483 overnight at 4°C. Antibody binding was detected using ab175745 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)Anti-beta Actin antibody - Loading Control (ab8229) at 1 µg/ml + HeLa Whole Cell Lysate at 10 µg
Secondary
Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745) at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 42 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with ab8229 overnight at 4°C. Antibody binding was detected using ab175745 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献
ab175745 は論文での使用が確認できていません。
