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  1. Link

    donkey-fab2-sheep-igg-hl-pe-preadsorbed-ab7009.pdf

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Immunology Immunoglobulins Heavy Chain IgG
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Donkey F(ab')2 Anti-Sheep IgG H&L (PE) preadsorbed (ab7009)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (1)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

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出荷および受注について

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Donkey F(ab')2 Anti-Sheep IgG H&L (PE) preadsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt

こちらの製品もご検討ください

標識
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)
ブロッキング
Product image
Normal Donkey Serum (ab7475)

関連製品

製品の概要

  • 製品名

    Donkey F(ab')2 Anti-Sheep IgG H&L (PE) preadsorbed
    IgG 二次抗体 製品一覧
  • 由来種

    Donkey
  • ターゲット生物種

    Sheep
  • アプリケーション

    適用あり: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • 吸着処理血清


    Chicken, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • 免疫原

    Sheep IgG whole molecule

  • 標識

    PE. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Prepared from monospecific antiserum by immunoaffinity chromatography using sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • 特記事項(標識)

    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 特記事項


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究分野

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Sheep
    • IgG
    • Fluorophore
    • Phycoerythrin

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7009の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/200.
IHC-Fr
1/50 - 1/200.
Immunomicroscopy
1/50 - 1/200.
Flow Cyt
1/100 - 1/250.
特記事項
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/200.
IHC-Fr
1/50 - 1/200.
Immunomicroscopy
1/50 - 1/200.
Flow Cyt
1/100 - 1/250.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (1)

ab7009 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7009 は 1 報の論文で使用されています。

  • Tanaka Y  et al. Sustained macroscopic engraftment of cynomolgus embryonic stem cells in xenogeneic large animals after in utero transplantation. Stem Cells Dev 17:367-81 (2008). Flow Cyt . PubMed: 18447651

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Question

Antibody seems not to perform recognizing serum opsonised beads or lateral flow capture lines that get a strong signal when using monoclonal anti sheep FITC antibody. Antibodies in lateral flow were also not recognised by anti-lambda ovine antibody so potentially the polyclonal recognises preferentially lambda which would be correct as sheep have 99% lambda antibodies. It would be worthwhile testing this out and put a warning onto the use of the antibody.

Read More

Abcam community

Verified customer

Asked on Mar 23 2012

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody in lateral flow experiments.

We appreciate your valuable feedback. Regrettably, this antibody has not been tested in lateral flow, so we would unfortunately not be able to guarantee how well it will work in this case.

I would like to investigate this particular case for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I would appreciate if you are able to provide some further information on the experiments:

1. Please provide more detail regarding what type of samples were tested?
2. What was the protocol used?
3. I would appreciate if you could also provide any images which would help us to assess the results.

Also, could you confirm if the antibody has been tried in a tested application? I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytometry. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

Thank you for your time and cooperation. We look forward to receiving the requested information.

Read More

Abcam Scientific Support

Answered on Mar 23 2012

Question

Thank you for your response, please see below the response from the customer;

I thought I had explained myself clearly. I am well aware that these are donkey derived antibodies! I am using them against sheeppolyclonal antibodies (purified to contain onlyIgG). The sheep polyclonals are just attached to beads. The beads are then measured for PE fluorescence. The fact is that the same product made byanother company seems to work quite well whereas the Abcam one does not.

I'm not sure what other information I can give, but I'm pretty disappointed with that response from Abcam.

Kind regards,

Read More

Abcam community

Verified customer

Asked on May 08 2012

Answer

Thank you for your response and the further clarification.

I am afraid this information was not provided in the initial e-mail.

Your credit note ID is 20806.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Read More

Abcam Scientific Support

Answered on May 08 2012

Question

My apologies! Sorry that was pretty silly of me to forget the catalogue number .. K


It is ab7009 Donkey F(ab')2 polyclonal Secondary Antibody to Sheep IgG - H&L (PE), pre-adsorbed

Please see below for an email we received from a customer;

I have tested this product (twice) and was disappointed with the results. When compared to a 4 year old vial of the equivalent Santa Cruz product, the Abcam antibody produced about 1/10th the signal. The application was a validation of sheep polyclonal antibody coupled beads, used for and read by a Bio-plex instrument (basically measuring the amount of sheep antibodies bound to the beads). All conditions in the experiment were the same, including test sample and antibody concentration.


Lot #: GR38595

Sapphire order number: PO-13364
Abcam invoice number : 1315698B



Sorry again!



Hope that helps!



Kind regards,

Read More

Abcam community

Verified customer

Asked on May 04 2012

Answer

My colleague is out of office and she has asked me to look after her customers in her absence.

Thank you for response and for confirming the product code, the batch and the order number> I am a bit confused about this enquiry since ab7009 is a donkey polyclonal antibody and not sheep, so it contains donkey IgG (F(ab')2) - fragment.

It was raised against sheep IgG so the immunogne is sheep IgG whole molecule but the actual product consists of donkey immunoglobulins.

Furthermore, ab7009 has not been tested in IP or any separation technique which requires beads. It has been characterized ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Abcam Scientific Support

Answered on May 04 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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