Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)

Submit a review Q&A (4)References (19)

Key features and details

  • Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, Immunomicroscopy, Flow Cyt

製品の概要

  • 製品名

    Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed
    IgG 二次抗体 製品一覧

  • 製品の詳細

    Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed

  • 由来種

    Donkey

  • ターゲット生物種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, ICC/IF, Immunomicroscopy, Flow Cytmore details

  • 吸着処理血清


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details

  • 免疫原

    Rabbit IgG whole molecule

  • 標識

    PE. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Prepared from monospecific antiserum by immunoaffinity chromatography using rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins

  • 特記事項（標識）

    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 特記事項


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab7007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200.
ICC/IF 1/100 - 1/250.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.

画像

  • Flow Cytometry - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)
    Flow Cytometry - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)
    Ab7007 was used with the primary antibody ab18203 in Flow Cyt. See Abreview on ab18203.
  • Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)
    Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)
    Ab7007 was used at dilution 1/250 with the primary antibody ab24182 in ICC/IF. See the review on ab24182.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (19)

ab7007 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7007 は 19 報の論文で使用されています。

  • Amjadi F  et al. Distinct changes in the proteome profile of endometrial tissues in polycystic ovary syndrome compared with healthy fertile women. Reprod Biomed Online N/A:N/A (2018). WB, IHC-P . PubMed: 29729850
  • Vasilev DS  et al. Transient Morphological Alterations in the Hippocampus After Pentylenetetrazole-Induced Seizures in Rats. Neurochem Res N/A:N/A (2018). PubMed: 29936569
  • Xu YL  et al. MicroRNA-19 restores vascular endothelial cell function in lower limb ischemia-reperfusion injury through the KLF10-dependent TGF-ß1/Smad signaling pathway in rats. J Cell Biochem N/A:N/A (2018). PubMed: 29953651
  • da Silva Lima F  et al. An insight into the role of magnesium in the immunomodulatory properties of mesenchymal stem cells. J Nutr Biochem 55:200-208 (2018). Flow Cyt, ICC/IF . PubMed: 29554498
  • Vaez SA  et al. The cardiac niche role in cardiomyocyte differentiation of rat bone marrow-derived stromal cells: comparison between static and microfluidic cell culture methods. EXCLI J 17:762-774 (2018). PubMed: 30190666
View all Publications for this product

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Question

Customer kindly contacted us to inquire about the immunogen sequence for ab56215.

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Answer

Thank you for contacting us today regarding secondary antibodies.

I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is conjugated a different fluorochome so that you may chose the one which best fits your experiments. Please let me know if you have any questions.

AMCA:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-AMCA-ab123435.html

PE:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-PE-pre-adsorbed-ab72465.html

DyLight 594:https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-594-pre-adsorbed-ab98500.html

Texas Red:https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7088.html

APC:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-Allophycocyanin-pre-adsorbed-ab130805.html

If testing live cells we also recommend cell viability controls such as 7-AAD orPropidium Iodide.Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AADCell-Mediated Cytotoxicity Assay Kit.

Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

7-AAD Kit:https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html



In reviewing the datasheet for this product I did note that it has not been tested in Flow Cytometry. As such we cannot guarantee ab56212 in that application. However by participating in our AbTrial program, you can now use our products in an untested application or species without financial risk.



Simply follow these easy steps below to take part in our AbTrial Program:

1. Reply to this e-mail to let me know that you would like to proceed and test this product in Flow Cytometry.

2. I will email you aninactivepersonal discount code for the value of the product.

3. Purchase and test the product at regular price.

4. https://www.abcam.com/abreviews with you results noting your AbTrial discount code in the additional notes section.

5. Once the Abreview is submitted, the discount code will becomeactive.

6. Apply your discount code on your next order to receive that value off.

More information including the terms and conditions about this program may be found at: https://www.abcam.com/AbTrial

I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

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Question

High FACS signal from secondary-only control. What modification do you suggest?

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Answer

Thank you for your reply. Your protocol looks correct. The only possible issues I see are the concentration of the secondary antibody that you incubate with the cells but more importantly, the lack of saponin in the wash buffer. The saponin permeabilization is reversible, so your washes will not be effective if they do not include saponin. I am guessing that, without the saponin in the wash buffer, the membrane is not permeabilized, and the secondary antibody PE conjugate is retained in the cells.

For the secondary antibody, are you adding 10ul of the stock antibody solution, undiluted? If so, then the concentration is too high. For FACS, the datasheet recommends that the cells receive should be 1/100 - 1/250, but I suggest diluting it even more, given the relatively low number of cells in your suspension (compared to 0.5e6 - 1e6 per 100ul) . I recommend, if you are not already doing this, diluting the ab7007 to a dilution of 1/50 with the buffer you use to dilute the primary antibody (with saponin) and then add 10ul of this to your 100ul cell suspension. This will be a final dilution of 1/500.

The primary antibody should be at a final dilution of 1/30.

I do not think the FcR blocker is the problem. You may want to try a test without it, just one tube of cells, to see if it is effective or if it is somehow contributing to the unusually high signal. That may be the case if the secondary antibody has some affinity for the blocker. Unlikely, but possible.

Please let me know if you have any questions or if the problem persists.

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Question

Dear Sirt’s Reference to the above subject (Glucocorticoid Receptor; ab9568 or ab109022) .. the customer need to work it by Flow Cytometer.. and I think this type of work need a secondary anti-body. But the TDS (technical data sheet) on the abcam website doesn’t suggest any kind of secondary anti-body. So please suggest a secondary anti-body by color of PE (Phycoerythrin) work with ab9568 or ab109022 by Flow Cytometer. thanks Sincerely yours,      

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Answer

Thank you for your enquiry and interest in our products. In order to be able to search the compatible secondary antibodies, first  it is important to consider the isotypes of these two primary antibodies: ab9568 - mouse IgG1 kappa ab109022 - rabbit IgG1 I would advise you and your customer to use our recently developed advanced search facilities for secondary antibodies: https://www.abcam.com/index.html?pageconfig=productmap&cl=918 I have conducted some search for you and you can find some compatible antibodies: For ab9568: ab99605 - Rat monoclonal [SB77e] Secondary Antibody to Mouse IgG1 (PE), pre-adsorbed ab99912 - Goat polyclonal Secondary Antibody to Mouse IgG1 (PE) For ab109022: ab97070 - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed -Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), ab98441- Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), ab98494- Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed, ab7007- Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed, ab72465 - Goat polyclonal Secondary Antibody to Rabbit IgG (PE), pre-adsorbed   I hope this helps and if I can assist further, please do not hesitate to contact me.    

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Question

Please confirm that this antibody has been tested for application in IHC-frozen sections, and if yes, what dilution is recommended.

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Answer

Thank you for your phone call. I checked with the originator of this antibody and they informed me that it was not specifically tested for application in IHC-frozen sections or formalin-fixed paraffin embedded sections. I have updated our datasheet to reflect this. It was tested in Immunomicroscopy and Flow Cytometry / FACS and can be used in other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. It should work, however, for IHC-frozen sections and the originator recommended using a 1:500 dilution. If you want to return the antibody, please let me know and I will inform you as to how to proceed.

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