Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150072)

製品の概要

  • 製品名

    Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed
    IgG 二次抗体 製品一覧
  • 由来種

    Donkey
  • ターゲット生物種

    Rabbit
  • 特異性

    Reacts with the heavy and light chains of rabbit IgG as demonstrated by ELISA; may also react with other species and with the light chains of other rabbit immunoglobulins.
  • アプリケーション

    適用あり: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • 吸着処理血清


    Human, Mouse, Rat more details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 標識

    Alexa Fluor® 594. Ex: 590nm, Em: 617nm

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • バッファー

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol, 68% PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 特記事項

    This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab150072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.
ELISA Use at an assay dependent concentration. Use at an assay dependent dilution
IHC-P Use at an assay dependent concentration. Use at an assay dependent dilution
Flow Cyt 1/2000.

画像

  • ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1µg/ml) overnight at +4°C. The secondary antibody (orange) was ab150072, Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 594), pre-adsorbed, used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The secondary antibody only control displays negative staining, as expected.

参考文献

This product has been referenced in:

  • Volpi S  et al. EXTL3 mutations cause skeletal dysplasia, immune deficiency, and developmental delay. J Exp Med 214:623-637 (2017). Read more (PubMed: 28148688) »
  • Ku T  et al. Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues. Nat Biotechnol 34:973-81 (2016). Read more (PubMed: 27454740) »
See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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