アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    donkey-fab2-human-igg-hl-pe-pre-adsorbed-ab7005.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Immunoglobulins Heavy Chain IgG
Share by email

Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab7005)

  • Datasheet
  • SDS
Submit a review Q&A (4)References (1)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Donkey
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt

こちらの製品もご検討ください

標識
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)
ブロッキング
Product image
Normal Donkey Serum (ab7475)

関連製品

製品の概要

  • 製品名

    Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
    IgG 二次抗体 製品一覧
  • 由来種

    Donkey
  • ターゲット生物種

    Human
  • アプリケーション

    適用あり: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • 吸着処理血清


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep
    To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
    more details
  • 免疫原

    Human IgG whole molecule

  • 標識

    PE. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • 特記事項(標識)

    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • ポリ/モノ

    ポリクローナル
  • 特記事項


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究分野

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Human
    • IgG
    • Fluorophore
    • Phycoerythrin

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7005の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/200.
IHC-Fr
1/50 - 1/200.
Immunomicroscopy
1/50 - 1/200.
Flow Cyt
1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
Lesser amounts of reagent may be sufficient for staining.
特記事項
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/200.
IHC-Fr
1/50 - 1/200.
Immunomicroscopy
1/50 - 1/200.
Flow Cyt
1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
Lesser amounts of reagent may be sufficient for staining.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (1)

ab7005 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7005 は 1 報の論文で使用されています。

  • Zhang Y  et al. Anti-hypoglycemic and hepatocyte-protective effects of hyperoside from Zanthoxylum bungeanum leaves in mice with high-carbohydrate/high-fat diet and alloxan-induced diabetes. Int J Mol Med 41:77-86 (2018). PubMed: 29115390

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-4 of 4 Abreviews or Q&A

Question

We have a customer who is experiencing troubles with your Human IgG antibody (ab7005). Please refer to below: ?We have purchased this antibody on 2 occasions now and have had no success in detecting PE fluorescence by flow cytometry in our IgG expressing cells. I note that the Immunogen is "Human IgG whole molecule" and I would like some clarification whether this means the antibody specifically recognizes the Fab portion (or hypervariable) portion of our IgG immunoglobulin alone. I also note that the specification sheet denotes under Relevance ".. absence of F(c) mediated binding" and therefore assume that this means that the antibody does not recognise the Fc portion of our secreted IgG immunoglobulin. Essentially, I am trying to detect a membrane bound IgG immunoglobulin, however I am not sure which domain (Fc or Fab) of our IgG molecule are exposed to the extracellular environment and as such whether using ab7005 is preferential to the hypervariable /Fab region only Clearly, if this is the case then we would need antibody which recognises the Fc portion in order to test our hypothesis.?. If you require any further information please do not hesitate to contact me.

Read More

Abcam community

Verified customer

Asked on Nov 09 2004

Answer

I just received this information from the source of the antibody: 1. The immunogen of Human IgG whole molecule means the antibody recognizes any epitope on the whole human IgG, not just Fab portion. 2. "..absence of F(c) mediated binding" means only F(ab')2 portion of the antibody will bind to epitopes of antigen including epitopes on the Fc portion. There is no binding to Fc receptors existing on cell surfaces. The antibody conjugated to PE is extensively cross adsorbed against serum proteins from 10 different species. Each cross that is performed removes more and more reactive antibodies from the pool until a much more narrow collection of antibody remains. While this is great for multiple label experiments, ie. to lower background against horse or goat or rabbit etc., it tends to lower the reactivity of the reagent against some specific epitopes of multiple-epitope antigen and some of the human subclass monoclonals too. I hope this information helps, please do not hesitate to contact us if you need further details,

Read More

Abcam Scientific Support

Answered on Nov 10 2004

Question

We have a customer who is experiencing troubles with your Human IgG antibody (ab7005). Please refer to below: ?We have purchased this antibody on 2 occasions now and have had no success in detecting PE fluorescence by flow cytometry in our IgG expressing cells. I note that the Immunogen is "Human IgG whole molecule" and I would like some clarification whether this means the antibody specifically recognizes the Fab portion (or hypervariable) portion of our IgG immunoglobulin alone. I also note that the specification sheet denotes under Relevance ".. absence of F(c) mediated binding" and therefore assume that this means that the antibody does not recognise the Fc portion of our secreted IgG immunoglobulin. Essentially, I am trying to detect a membrane bound IgG immunoglobulin, however I am not sure which domain (Fc or Fab) of our IgG molecule are exposed to the extracellular environment and as such whether using ab7005 is preferential to the hypervariable /Fab region only Clearly, if this is the case then we would need antibody which recognises the Fc portion in order to test our hypothesis.?. If you require any further information please do not hesitate to contact me.

Read More

Abcam community

Verified customer

Asked on Nov 09 2004

Answer

I just received this information from the source of the antibody: 1. The immunogen of Human IgG whole molecule means the antibody recognizes any epitope on the whole human IgG, not just Fab portion. 2. "..absence of F(c) mediated binding" means only F(ab')2 portion of the antibody will bind to epitopes of antigen including epitopes on the Fc portion. There is no binding to Fc receptors existing on cell surfaces. The antibody conjugated to PE is extensively cross adsorbed against serum proteins from 10 different species. Each cross that is performed removes more and more reactive antibodies from the pool until a much more narrow collection of antibody remains. While this is great for multiple label experiments, ie. to lower background against horse or goat or rabbit etc., it tends to lower the reactivity of the reagent against some specific epitopes of multiple-epitope antigen and some of the human subclass monoclonals too. I hope this information helps, please do not hesitate to contact us if you need further details,

Read More

Abcam Scientific Support

Answered on Nov 10 2004

Question

How many micrograms are in the anti-IgG ab 7005....it gives microlitres but not micrograms....thanks

Read More

Abcam community

Verified customer

Asked on Oct 13 2004

Answer

Thank you for your enquiry. The concentration of this product is 0.5 mg/mL. The total would be 500ug.

Read More

Abcam Scientific Support

Answered on Oct 18 2004

Question

Customer would like to know the Phycobiliprotein Concentration of ab7005, and what is the ratio of antibody to PE for ab7005 and ab7006.

Read More

Abcam community

Verified customer

Asked on Nov 13 2003

Answer

The Phycobiliprotein concentration of ab7005 is 0.5mg/mL. For both ab7005 and ab7006, there is one PE molecule with at least one molecule of active antibody. If you have any more questions, please contact us again.

Read More

Abcam Scientific Support

Answered on Nov 17 2003

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

技術情報や製品情報をメールで受け取る 登録
製品・研究分野別
  • 癌
  • 循環器
  • 細胞生物学
  • 発生
  • エピジェネティクス&核内情報
  • 免疫学
  • メタボリズム
  • 感染症
  • ニューロサイエンス
  • シグナル伝達
  • ステムセル
製品・タイプ別
  • 一次抗体
  • 二次抗体
  • バイオケミカルズ
  • アイソタイプ・コントロール
  • マルチカラー・セレクター
  • キット
  • ローディング・コントロール
  • ライゼート
  • ペプチド
  • タンパク質
  • スライド
  • タグ抗体・細胞マーカー
  • その他試薬
サービス&サポート
  • サポート
  • よくあるお問い合わせ
  • プロトコール&トラブルシュート
  • ご購入に関して
  • お問い合わせフォーム
  • トレーニング
  • ターゲット別に見る
アブカムについて
  • 企業ウェブサイト
  • 投資家の皆様へ
  • 企業情報
  • 採用情報
  • アブカムについて
イベント
  • 展示会
  • カンファレンス
International websites
  • abcam.com
  • abcam.cn

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
© 1998-2022 Abcam plc. All rights reserved.