Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed (ab7005)

Submit a review Q&A (4)References (1)

Key features and details

  • Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Host species: Donkey
  • Suitable for: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cyt

製品の概要

  • 製品名

    Donkey F(ab')2 Anti-Human IgG - H&L (PE), pre-adsorbed
    IgG 二次抗体 製品一覧

  • 由来種

    Donkey

  • ターゲット生物種

    Human

  • アプリケーション

    適用あり: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details

  • 吸着処理血清


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Mouse, Rabbit, Rat, Sheep more details

  • 免疫原

    Human IgG whole molecule

  • 標識

    PE. Ex: 488nm, Em: 575nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    Prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation

  • 特記事項（標識）

    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8

  • ポリ/モノ

    ポリクローナル

  • 特記事項


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7005の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/200.
IHC-Fr
1/50 - 1/200.
Immunomicroscopy
1/50 - 1/200.
Flow Cyt
1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
Lesser amounts of reagent may be sufficient for staining.
特記事項
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/200.
IHC-Fr
1/50 - 1/200.
Immunomicroscopy
1/50 - 1/200.
Flow Cyt
1/100 - 1/250.
The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.
Lesser amounts of reagent may be sufficient for staining.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab7005 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7005 は 1 報の論文で使用されています。

  • Zhang Y  et al. Anti-hypoglycemic and hepatocyte-protective effects of hyperoside from Zanthoxylum bungeanum leaves in mice with high-carbohydrate/high-fat diet and alloxan-induced diabetes. Int J Mol Med 41:77-86 (2018). PubMed: 29115390

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Question

We have a customer who is experiencing troubles with your Human IgG antibody (ab7005). Please refer to below: ?We have purchased this antibody on 2 occasions now and have had no success in detecting PE fluorescence by flow cytometry in our IgG expressing cells. I note that the Immunogen is "Human IgG whole molecule" and I would like some clarification whether this means the antibody specifically recognizes the Fab portion (or hypervariable) portion of our IgG immunoglobulin alone. I also note that the specification sheet denotes under Relevance ".. absence of F(c) mediated binding" and therefore assume that this means that the antibody does not recognise the Fc portion of our secreted IgG immunoglobulin. Essentially, I am trying to detect a membrane bound IgG immunoglobulin, however I am not sure which domain (Fc or Fab) of our IgG molecule are exposed to the extracellular environment and as such whether using ab7005 is preferential to the hypervariable /Fab region only Clearly, if this is the case then we would need antibody which recognises the Fc portion in order to test our hypothesis.?. If you require any further information please do not hesitate to contact me.

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Answer

I just received this information from the source of the antibody: 1. The immunogen of Human IgG whole molecule means the antibody recognizes any epitope on the whole human IgG, not just Fab portion. 2. "..absence of F(c) mediated binding" means only F(ab')2 portion of the antibody will bind to epitopes of antigen including epitopes on the Fc portion. There is no binding to Fc receptors existing on cell surfaces. The antibody conjugated to PE is extensively cross adsorbed against serum proteins from 10 different species. Each cross that is performed removes more and more reactive antibodies from the pool until a much more narrow collection of antibody remains. While this is great for multiple label experiments, ie. to lower background against horse or goat or rabbit etc., it tends to lower the reactivity of the reagent against some specific epitopes of multiple-epitope antigen and some of the human subclass monoclonals too. I hope this information helps, please do not hesitate to contact us if you need further details,

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Question

We have a customer who is experiencing troubles with your Human IgG antibody (ab7005). Please refer to below: ?We have purchased this antibody on 2 occasions now and have had no success in detecting PE fluorescence by flow cytometry in our IgG expressing cells. I note that the Immunogen is "Human IgG whole molecule" and I would like some clarification whether this means the antibody specifically recognizes the Fab portion (or hypervariable) portion of our IgG immunoglobulin alone. I also note that the specification sheet denotes under Relevance ".. absence of F(c) mediated binding" and therefore assume that this means that the antibody does not recognise the Fc portion of our secreted IgG immunoglobulin. Essentially, I am trying to detect a membrane bound IgG immunoglobulin, however I am not sure which domain (Fc or Fab) of our IgG molecule are exposed to the extracellular environment and as such whether using ab7005 is preferential to the hypervariable /Fab region only Clearly, if this is the case then we would need antibody which recognises the Fc portion in order to test our hypothesis.?. If you require any further information please do not hesitate to contact me.

Read More
Answer

I just received this information from the source of the antibody: 1. The immunogen of Human IgG whole molecule means the antibody recognizes any epitope on the whole human IgG, not just Fab portion. 2. "..absence of F(c) mediated binding" means only F(ab')2 portion of the antibody will bind to epitopes of antigen including epitopes on the Fc portion. There is no binding to Fc receptors existing on cell surfaces. The antibody conjugated to PE is extensively cross adsorbed against serum proteins from 10 different species. Each cross that is performed removes more and more reactive antibodies from the pool until a much more narrow collection of antibody remains. While this is great for multiple label experiments, ie. to lower background against horse or goat or rabbit etc., it tends to lower the reactivity of the reagent against some specific epitopes of multiple-epitope antigen and some of the human subclass monoclonals too. I hope this information helps, please do not hesitate to contact us if you need further details,

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Question

How many micrograms are in the anti-IgG ab 7005....it gives microlitres but not micrograms....thanks

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Answer

Thank you for your enquiry. The concentration of this product is 0.5 mg/mL. The total would be 500ug.

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Question

Customer would like to know the Phycobiliprotein Concentration of ab7005, and what is the ratio of antibody to PE for ab7005 and ab7006.

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Answer

The Phycobiliprotein concentration of ab7005 is 0.5mg/mL. For both ab7005 and ab7006, there is one PE molecule with at least one molecule of active antibody. If you have any more questions, please contact us again.

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