Key features and details
- Goat polyclonal to DOK3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
DOK3 一次抗体 製品一覧
製品の詳細Goat polyclonal to DOK3
アプリケーション適用あり: WBmore details
種交差性交差種: Mouse, Human
- Human PBMC lysate.
特記事項GenBank Accession Number – NP_079148.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab1357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution >1/32000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.
組織特異性Expressed in spleen.
配列類似性Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
ドメインPTB domain mediates receptor interaction.
On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.
細胞内局在Cytoplasm. Cell membrane.
- Information by UniProt
- Docking protein 3 antibody
- DOK 3 antibody
- DOK Like Protein antibody
Anti-DOK3 antibody (ab1357) at 0.1 µg/ml + Human PBMC lysate (RIPA buffer) at 35 µg
Predicted band size: 57 kDa
ab1357 staining (1Primary incubated for 1 hour. Detected by chemiluminescence.
µg/ml) of Human PBMC lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
All lanes : Anti-DOK3 antibody (ab1357) at 2 µg/ml
Lane 1 : Human Bone Marrow lysates
Lane 2 : Peripheral blood leukocyte lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 57 kDa
ab1357 は 3 報の論文で使用されています。
- Bounab Y et al. Proteomic analysis of the SH2domain-containing leukocyte protein of 76 kDa (SLP76) interactome. Mol Cell Proteomics 12:2874-89 (2013). Mouse . PubMed: 23820730
- Selimovic D et al. Apoptosis related protein-1 triggers melanoma cell death via interaction with the juxtamembrane region of p75 neurotrophin receptor. J Cell Mol Med 16:349-61 (2012). PubMed: 21418516
- Heckel T et al. Src-dependent repression of ARF6 is required to maintain podosome-rich sealing zones in bone-digesting osteoclasts. Proc Natl Acad Sci U S A 106:1451-6 (2009). WB ; Mouse . PubMed: 19164586