Anti-DOK3 抗体 (ab1357)

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Western blot - Anti-DOK3 antibody (ab1357)
  • Western blot - Anti-DOK3 antibody (ab1357)

Key features and details

  • Goat polyclonal to DOK3
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-DOK3 antibody
    DOK3 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to DOK3

  • 由来種

    Goat

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide:

    TRERRKGPAPCDRP

    , corresponding to C terminal amino acids 483-490 of Human DOK3.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Human PBMC lysate.

  • 特記事項

    GenBank Accession Number – NP_079148.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab1357の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
特記事項
  • 追加情報
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 60-65 kDa (predicted molecular weight: 57 kDa).
    Peptide ELISA: antibody detection limit dilution >1/32000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能

      DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.

    • 組織特異性

      Expressed in spleen.

    • 配列類似性

      Belongs to the DOK family. Type A subfamily.
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.

    • ドメイン

      PTB domain mediates receptor interaction.

    • 翻訳後修飾

      Constitutively tyrosine-phosphorylated.
      On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.

    • 細胞内局在

      Cytoplasm. Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Docking protein 3 antibody
        • DOK 3 antibody
        • DOK Like Protein antibody
        • Dok3 antibody
        • DOK3_HUMAN antibody
        • DOKL Pending antibody
        • Downstream of tyrosine kinase 3 antibody
        • p62 DOK Like Protein antibody
        see all

      画像

      • Western blot - Anti-DOK3 antibody (ab1357)
        Western blot - Anti-DOK3 antibody (ab1357)
        Anti-DOK3 antibody (ab1357) at 0.1 µg/ml + Human PBMC lysate (RIPA buffer) at 35 µg

        Predicted band size: 57 kDa



        ab1357 staining (1µg/ml) of Human PBMC lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

        Primary incubated for 1 hour. Detected by chemiluminescence.
      • Western blot - Anti-DOK3 antibody (ab1357)
        Western blot - Anti-DOK3 antibody (ab1357)
        All lanes : Anti-DOK3 antibody (ab1357) at 2 µg/ml

        Lane 1 : Human Bone Marrow lysates
        Lane 2 : Peripheral blood leukocyte lysates

        Lysates/proteins at 35 µg per lane.

        Predicted band size: 57 kDa

      参考文献 (3)

      ab1357 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab1357 は 3 報の論文で使用されています。

      • Bounab Y  et al. Proteomic analysis of the SH2domain-containing leukocyte protein of 76 kDa (SLP76) interactome. Mol Cell Proteomics 12:2874-89 (2013). Mouse . PubMed: 23820730
      • Selimovic D  et al. Apoptosis related protein-1 triggers melanoma cell death via interaction with the juxtamembrane region of p75 neurotrophin receptor. J Cell Mol Med 16:349-61 (2012). PubMed: 21418516
      • Heckel T  et al. Src-dependent repression of ARF6 is required to maintain podosome-rich sealing zones in bone-digesting osteoclasts. Proc Natl Acad Sci U S A 106:1451-6 (2009). WB ; Mouse . PubMed: 19164586

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