Anti-DOK3 抗体 (ab1357)
製品の概要
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製品名
Anti-DOK3 antibody
DOK3 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DOK3 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide:
TRERRKGPAPCDRP
, corresponding to C terminal amino acids 483-490 of Human DOK3. -
ポジティブ･コントロール
- Human PBMC lysate.
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特記事項
GenBank Accession Number – NP_079148.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
追加情報
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 60-65 kDa (predicted molecular weight: 57 kDa).
Peptide ELISA: antibody detection limit dilution >1/32000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: antibody detection limit dilution >1/32000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function. -
組織特異性
Expressed in spleen. -
配列類似性
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
ドメイン
PTB domain mediates receptor interaction. -
翻訳後修飾
Constitutively tyrosine-phosphorylated.
On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase. -
細胞内局在
Cytoplasm. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 79930 Human
- Entrez Gene: 27261 Mouse
- Entrez Gene: 306760 Rat
- Omim: 611435 Human
- SwissProt: Q7L591 Human
- SwissProt: Q9QZK7 Mouse
- SwissProt: B2RYG7 Rat
- Unigene: 720849 Human
see all -
別名
- Docking protein 3 antibody
- DOK 3 antibody
- DOK Like Protein antibody
see all
画像
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Western blot - Anti-DOK3 antibody (ab1357)Anti-DOK3 antibody (ab1357) at 0.1 µg/ml + Human PBMC lysate (RIPA buffer) at 35 µg
Predicted band size: 57 kDaab1357 staining (1
Primary incubated for 1 hour. Detected by chemiluminescence.µ g/ml) of Human PBMC lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Western blot - Anti-DOK3 antibody (ab1357)All lanes : Anti-DOK3 antibody (ab1357) at 2 µg/ml
Lane 1 : Human Bone Marrow lysates
Lane 2 : Peripheral blood leukocyte lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 57 kDa
プロトコール
データシートおよび資料
参考文献
This product has been referenced in:
- Bounab Y et al. Proteomic analysis of the SH2domain-containing leukocyte protein of 76 kDa (SLP76) interactome. Mol Cell Proteomics 12:2874-89 (2013). Mouse . Read more (PubMed: 23820730) »
- Heckel T et al. Src-dependent repression of ARF6 is required to maintain podosome-rich sealing zones in bone-digesting osteoclasts. Proc Natl Acad Sci U S A 106:1451-6 (2009). WB ; Mouse . Read more (PubMed: 19164586) »
