Anti-DOK3 抗体 (ab1357)

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Western blot - Anti-DOK3 antibody (ab1357)
  • Western blot - Anti-DOK3 antibody (ab1357)

Key features and details

  • Goat polyclonal to DOK3
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-DOK3 antibody
    DOK3 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to DOK3

  • 由来種

    Goat

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide:

    TRERRKGPAPCDRP

    , corresponding to C terminal amino acids 483-490 of Human DOK3.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Human PBMC lysate.

  • 特記事項

    GenBank Accession Number – NP_079148.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 60-65 kDa (predicted molecular weight: 57 kDa).
    Peptide ELISA: antibody detection limit dilution >1/32000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能

      DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.

    • 組織特異性

      Expressed in spleen.

    • 配列類似性

      Belongs to the DOK family. Type A subfamily.
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.

    • ドメイン

      PTB domain mediates receptor interaction.

    • 翻訳後修飾

      Constitutively tyrosine-phosphorylated.
      On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.

    • 細胞内局在

      Cytoplasm. Cell membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • Docking protein 3 antibody
      • DOK 3 antibody
      • DOK Like Protein antibody
      • Dok3 antibody
      • DOK3_HUMAN antibody
      • DOKL Pending antibody
      • Downstream of tyrosine kinase 3 antibody
      • p62 DOK Like Protein antibody
      see all

    画像

    • Western blot - Anti-DOK3 antibody (ab1357)
      Western blot - Anti-DOK3 antibody (ab1357)
      Anti-DOK3 antibody (ab1357) at 0.1 µg/ml + Human PBMC lysate (RIPA buffer) at 35 µg

      Predicted band size: 57 kDa



      ab1357 staining (1µg/ml) of Human PBMC lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

      Primary incubated for 1 hour. Detected by chemiluminescence.
    • Western blot - Anti-DOK3 antibody (ab1357)
      Western blot - Anti-DOK3 antibody (ab1357)
      All lanes : Anti-DOK3 antibody (ab1357) at 2 µg/ml

      Lane 1 : Human Bone Marrow lysates
      Lane 2 : Peripheral blood leukocyte lysates

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 57 kDa

    参考文献 (3)

    ab1357 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1357 は 3 報の論文で使用されています。

    • Bounab Y  et al. Proteomic analysis of the SH2domain-containing leukocyte protein of 76 kDa (SLP76) interactome. Mol Cell Proteomics 12:2874-89 (2013). Mouse . PubMed: 23820730
    • Selimovic D  et al. Apoptosis related protein-1 triggers melanoma cell death via interaction with the juxtamembrane region of p75 neurotrophin receptor. J Cell Mol Med 16:349-61 (2012). PubMed: 21418516
    • Heckel T  et al. Src-dependent repression of ARF6 is required to maintain podosome-rich sealing zones in bone-digesting osteoclasts. Proc Natl Acad Sci U S A 106:1451-6 (2009). WB ; Mouse . PubMed: 19164586

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