Anti-DOK3 抗体 (ab1357)

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製品の概要

  • 製品名

    Anti-DOK3 antibody
    DOK3 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to DOK3

  • 由来種

    Goat

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide:

    TRERRKGPAPCDRP

    , corresponding to C terminal amino acids 483-490 of Human DOK3.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Human PBMC lysate.

  • 特記事項

    GenBank Accession Number – NP_079148.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 60-65 kDa (predicted molecular weight: 57 kDa).
    Peptide ELISA: antibody detection limit dilution >1/32000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能

      DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate Abl function.

    • 組織特異性

      Expressed in spleen.

    • 配列類似性

      Belongs to the DOK family. Type A subfamily.
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.

    • ドメイン

      PTB domain mediates receptor interaction.

    • 翻訳後修飾

      Constitutively tyrosine-phosphorylated.
      On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.

    • 細胞内局在

      Cytoplasm. Cell membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • Docking protein 3 antibody
      • DOK 3 antibody
      • DOK Like Protein antibody
      • Dok3 antibody
      • DOK3_HUMAN antibody
      • DOKL Pending antibody
      • Downstream of tyrosine kinase 3 antibody
      • p62 DOK Like Protein antibody
      see all

    画像

    • Western blot - Anti-DOK3 antibody (ab1357)
      Western blot - Anti-DOK3 antibody (ab1357)
      Anti-DOK3 antibody (ab1357) at 0.1 µg/ml + Human PBMC lysate (RIPA buffer) at 35 µg

      Predicted band size: 57 kDa



      ab1357 staining (1µg/ml) of Human PBMC lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.

      Primary incubated for 1 hour. Detected by chemiluminescence.
    • Western blot - Anti-DOK3 antibody (ab1357)
      Western blot - Anti-DOK3 antibody (ab1357)
      All lanes : Anti-DOK3 antibody (ab1357) at 2 µg/ml

      Lane 1 : Human Bone Marrow lysates
      Lane 2 : Peripheral blood leukocyte lysates

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 57 kDa

    参考文献

    ab1357 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab1357 は 2 報の論文で使用されています。

    • Bounab Y  et al. Proteomic analysis of the SH2domain-containing leukocyte protein of 76 kDa (SLP76) interactome. Mol Cell Proteomics 12:2874-89 (2013). Mouse . PubMed: 23820730
    • Heckel T  et al. Src-dependent repression of ARF6 is required to maintain podosome-rich sealing zones in bone-digesting osteoclasts. Proc Natl Acad Sci U S A 106:1451-6 (2009). WB ; Mouse . PubMed: 19164586

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