Anti-DOK1 抗体 (ab8112)
Key features and details
- Rabbit polyclonal to DOK1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-DOK1 antibody
DOK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DOK1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to amino acids 425-439 of Human DOK1 (Peptide available as ab39756.)
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ポジティブ･コントロール
- Jurkat whole cell lysate
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特記事項
Tyrosine kinase substrate p62DOKSignals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptortyrosine kinases. Activated tyrosine kinases phosphorylatetheir substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56 dok.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptortyrosine kinases. Activated tyrosine kinases phosphorylatetheir substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56 dok. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8112の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.
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| ICC/IF |
Use a concentration of 2 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa. |
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ICC/IF
Use a concentration of 2 µg/ml. |
ターゲット情報
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機能
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. -
組織特異性
Expressed in pancreas, heart, leukocyte and spleen. Expressed in both resting and activated peripheral blood T-cells. -
配列類似性
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
ドメイン
The PTB domain mediates receptor interaction. -
翻訳後修飾
Constitutively tyrosine-phosphorylated.
Phosphorylated on tyrosine residues by the insulin receptor kinase. Results in the negative regulation of the insulin signaling pathway. -
細胞内局在
Cytoplasm and Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 1796 Human
- Omim: 602919 Human
- SwissProt: Q99704 Human
- Unigene: 103854 Human
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別名
- Docking protein 1 62kD antibody
- Docking protein 1 antibody
- DOK 1 antibody
see all
画像
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Western blot - Anti-DOK1 antibody (ab8112)All lanes : Anti-DOK1 antibody (ab8112) at 1 µg/ml
Lane 1 : Jurkat cell lysates
Lane 2 : THP-1 cell lysate
Observed band size: 62 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-DOK1 antibody (ab8112)ab8112 at 2µg/ml staining DOK1 in K562 cells by ICC/IF
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab8112 は 8 報の論文で使用されています。
- Sheng L et al. Overexpression of Fc?RIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. Int J Mol Med 46:1409-1422 (2020). PubMed: 32945349
- Friedrich T et al. Subcellular compartmentalization of docking protein-1 contributes to progression in colorectal cancer. EBioMedicine 8:159-72 (2016). IHC . PubMed: 27428427
- Siouda M et al. Epstein-Barr virus down-regulates tumor suppressor DOK1 expression. PLoS Pathog 10:e1004125 (2014). WB . PubMed: 24809689
- Barrett A et al. A crucial role for DOK1 in PDGF-BB-stimulated glioma cell invasion through p130Cas and Rap1 signalling. J Cell Sci 127:2647-58 (2014). PubMed: 24762811
- Celis-Gutierrez J et al. Dok1 and Dok2 proteins regulate natural killer cell development and function. EMBO J 33:1928-40 (2014). PubMed: 24963146
- Siouda M et al. Transcriptional regulation of the human tumor suppressor DOK1 by E2F1. Mol Cell Biol 32:4877-90 (2012). WB ; Human . PubMed: 23028047
- Schoenborn JR et al. Feedback circuits monitor and adjust Basal lck-dependent events in T cell receptor signaling. Sci Signal 4:ra59 (2011). PubMed: 21917715
- Park SK et al. Src-like adaptor protein (SLAP) is upregulated in antigen-stimulated mast cells and acts as a negative regulator. Mol Immunol 46:2133-9 (2009). WB . PubMed: 19371953
