Key features and details
- Rabbit polyclonal to Dnmt3a
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Dnmt3a 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Dnmt3a
アプリケーション適用あり: IHC-P, WBmore details
交差が予測される動物種: Mouse, Rat, Chicken
Synthetic peptide corresponding to Human Dnmt3a aa 457-486 conjugated to Keyhole Limpet Haemocyanin (KLH).
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.09% Sodium azide
Concentration information loading...
精製度Ammonium Sulphate Precipitation
特記事項（精製）This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Our Abpromise guarantee covers the use of ab4897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|WB||1/1000. Detects a band of approximately 101 kDa.|
機能Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
組織特異性Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
配列類似性Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.
ドメインThe PWWP domain is essential for targeting to pericentric heterochromatin.
翻訳後修飾Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
細胞内局在Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
- Information by UniProt
- DNA (cytosine 5) methyltransferase 3 alpha antibody
- DNA (cytosine 5) methyltransferase 3A antibody
- DNA (cytosine-5)-methyltransferase 3A antibody
ab4897 は 12 報の論文で使用されています。
- Yuan H et al. Hypomethylation of nerve growth factor (NGF) promotes binding of C/EBPa and contributes to inflammatory hyperalgesia in rats. J Neuroinflammation 17:34 (2020). PubMed: 31980031
- Li X et al. MCM3AP-AS1 KD Inhibits Proliferation, Invasion, and Migration of PCa Cells via DNMT1/DNMT3 (A/B) Methylation-Mediated Upregulation of NPY1R. Mol Ther Nucleic Acids 20:265-278 (2020). PubMed: 32193153
- Xu X et al. The long non-coding RNA Linc-GALH promotes hepatocellular carcinoma metastasis via epigenetically regulating Gankyrin. Cell Death Dis 10:86 (2019). PubMed: 30692513
- Akiyama N et al. Regulation of DNA methylation levels in the process of oral mucosal regeneration in a rat oral ulcer model. Histol Histopathol N/A:18147 (2019). PubMed: 31286466
- Ma M et al. Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1. Am J Transl Res 10:16-39 (2018). IHC-P ; Human . PubMed: 29422991