Anti-Dnmt3a 抗体 (ab2850)
Key features and details
- Rabbit polyclonal to Dnmt3a
- Suitable for: WB
- Reacts with: Mouse, Human, Drosophila melanogaster
- Isotype: IgG
製品の概要
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製品名
Anti-Dnmt3a antibody
Dnmt3a 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Dnmt3a -
由来種
Rabbit -
特異性
This antibody detects DNA methyltransferase 3a (Dnmt3a) from human and mouse tissues and cells as well as recombinant human Dnmt3a. This antibody does not detect full length recombinant human Dnmt3b or Dnmt1. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human, Drosophila melanogaster -
免疫原
Synthetic peptide corresponding to Human Dnmt3a aa 86-100.
Sequence:DLLPNGDLEKRSEPQ
(Peptide available asab4921)
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 101.5 kDa).Can be blocked with Dnmt3a peptide (ab4921). |
ターゲット情報
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機能
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity. -
組織特異性
Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. -
配列類似性
Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
ドメイン
The PWWP domain is essential for targeting to pericentric heterochromatin. -
翻訳後修飾
Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. -
細胞内局在
Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin. - Information by UniProt
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参照データベース
- Entrez Gene: 1788 Human
- Entrez Gene: 13435 Mouse
- Omim: 602769 Human
- SwissProt: Q9Y6K1 Human
- SwissProt: O88508 Mouse
- Unigene: 515840 Human
- Unigene: 5001 Mouse
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別名
- DNA (cytosine 5) methyltransferase 3 alpha antibody
- DNA (cytosine 5) methyltransferase 3A antibody
- DNA (cytosine-5)-methyltransferase 3A antibody
see all
画像
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Western blot - Anti-Dnmt3a antibody (ab2850)Image from Nucleic Acids Res. 2014 Jan; 42(2): 804–821. Fig 5D, doi: 10.1093/nar/gkt920 Reproduced under the creative commons licence. https://creativecommons.org/licenses/by/4.0/Western blot analysis of DNMT3a in HeLa silenced cells. Total cell extracts were prepared 48 h after electroporation and analyzed by immunoblot with ab2850.
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Western blot - Anti-Dnmt3a antibody (ab2850)Western blot of Dnmt3a on P19 cell nuclear extract using ab2850.
プロトコール
データシートおよび資料
参考文献 (67)
ab2850 は 67 報の論文で使用されています。
- Peng X et al. Long non-coding RNA TNRC6C-AS1 promotes methylation of STK4 to inhibit thyroid carcinoma cell apoptosis and autophagy via Hippo signalling pathway. J Cell Mol Med 24:304-316 (2020). PubMed: 31657132
- Zhang L et al. Crosstalk between histone modification and DNA methylation orchestrates the epigenetic regulation of the costimulatory factors, Tim-3 and galectin-9, in cervical cancer. Oncol Rep 42:2655-2669 (2019). PubMed: 31661141
- Ying Y et al. NLRP3 Inflammasome Activation by MicroRNA-495 Promoter Methylation May Contribute to the Progression of Acute Lung Injury. Mol Ther Nucleic Acids 18:801-814 (2019). PubMed: 31734560
- MacKay H et al. DNA methylation in AgRP neurons regulates voluntary exercise behavior in mice. Nat Commun 10:5364 (2019). PubMed: 31792207
- Chen H et al. Long non-coding XIST raises methylation of TIMP-3 promoter to regulate collagen degradation in osteoarthritic chondrocytes after tibial plateau fracture. Arthritis Res Ther 21:271 (2019). PubMed: 31815654
