Anti-Dnmt2 抗体 (ab272620)

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Western blot - Anti-Dnmt2 antibody (ab272620)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt2 antibody (ab272620)

Key features and details

  • Rabbit polyclonal to Dnmt2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Dnmt2 antibody
    Dnmt2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Dnmt2

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment corresponding to Human Dnmt2 aa 1-100.
    Database link: O14717

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: U-251 MG. IHC-P: Human colon tissue.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
WB
Human
All applications
Cow
アプリケーション Abreviews 特記事項
WB
Use a concentration of 0.04 - 0.4 µg/ml.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
Use a concentration of 0.04 - 0.4 µg/ml.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).

  • 組織特異性

    Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes.

  • 配列類似性

    Belongs to the C5-methyltransferase family.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • dDNMT antibody
      • DmMT 2 antibody
      • DmMT2 antibody
      • DNA (cytosine 5 ) methyltransferase 2 antibody
      • DNA (cytosine 5) methyltransferase like protein 2 antibody
      • DNA (cytosine-5)-methyltransferase-like protein 2 antibody
      • DNA 5 cytosine methyltransferase antibody
      • DNA methyltransferase 2 antibody
      • DNA methyltransferase homolog HsaIIP antibody
      • DNA MTase homolog HsaIIP antibody
      • Dnmt 2 antibody
      • Dnmt2 antibody
      • M.HsaIIP antibody
      • MHsaIIP antibody
      • nmt 2 antibody
      • nmt2 antibody
      • OTTHUMP00000045198 antibody
      • PuMet antibody
      • RNMT 1 antibody
      • RNMT1 antibody
      • TRDMT 1 antibody
      • TRDMT_HUMAN antibody
      • TRDMT1 antibody
      • tRNA (cytosine 5 ) methyltransferase antibody
      • tRNA (cytosine(38)-C(5))-methyltransferase antibody
      • tRNA aspartic acid methyltransferase 1 antibody
      • tRNA aspartic acid methyltransferase 1 variant 1 antibody
      • tRNA aspartic acid methyltransferase 1 variant 2 antibody
      • tRNA aspartic acid methyltransferase 1 variant 3 antibody
      • tRNA aspartic acid methyltransferase 1 variant 4 antibody
      • tRNA aspartic acid methyltransferase 1 variant 5 antibody
      • tRNA aspartic acid methyltransferase 1 variant 8 antibody
      see all

    画像

    • Western blot - Anti-Dnmt2 antibody (ab272620)
      Western blot - Anti-Dnmt2 antibody (ab272620)
      Lanes 2-3 : Anti-Dnmt2 antibody (ab272620) at 0.4 µg/ml

      Lane 1 : Molecular weight ladder
      Lane 2 : RT4
      Lane 3 : U-251 MG
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt2 antibody (ab272620)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt2 antibody (ab272620)

      Paraffin-embedded formalin-fixed Human colon tissue stained for Dnmt2 using ab272620 at 1/200 in immunohistochemical analysis.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272620 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272620 は論文での使用が確認できていません。

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