Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse colon cells)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
100000 cells
Specification
Mouse colon cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: anti mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 180 kDa
Additional data
Additional Notes
First band is control and second band is DNMT1 KD
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 26 2017