DNMT Activity Assay Kit (Fluorometric) (ab113468)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 3 hr 45 min
製品の概要
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製品名
DNMT Activity Assay Kit (Fluorometric)
DNMT キット 製品一覧 -
検出方法
Fluorescent -
アッセイタイプ
Enzyme activity -
全工程の試験時間
3h 45m -
種交差性
交差種: Mouse, Human, Plants
交差が予測される動物種: Mammals, Fungi -
製品の概要
DNMT (DNA methylases) is a family of enzymes responsible for the addition of methyl groups on the cytosine ring at the 5' position of a CpG dinucleotide. The DNMT family is required for the establishment and maintenance of DNA methylation patterns.
DNMT Activity Quantification Kit (Fluorometric) (ab113468) allows the user to fluorometrically measure DNA methyltransferase activity or inhibition at extremely fast speed on a 96-stripwell microplate. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Adhesive Covering Film 1 unit 1 unit Adomet, 50X 1 x 60µl 1 x 120µl Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 10µl Detection Antibody, 400 µg/mL 1 x 6µl 1 x 12µl DNMT Assay Buffer 1 x 4ml 1 x 8ml DNMT Enzyme Control, 50 µg/mL 1 x 6µl 1 x 12µl Enhancer Solution 1 x 6µl 1 x 12µl Fluoro Developer 1 x 6µl 1 x 12µl Fluoro Diluter 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 6µl 1 x 12µl -
研究分野
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab113468 は 1 報の論文で使用されています。
- Mao SQ et al. DNA G-quadruplex structures mold the DNA methylome. Nat Struct Mol Biol 25:951-957 (2018). PubMed: 30275516