Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7248] to DNAJA1
- Suitable for: WB, IP, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Rat, Human
製品名Anti-DNAJA1 antibody [EPR7248]
DNAJA1 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7248] to DNAJA1
アプリケーション適用あり: WB, IP, Flow Cytmore details
適用なし: ICC/IF or IHC-P
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human DNAJA1 aa 350-450 (C terminal). The exact sequence is proprietary.
- WB: HEK293T, Jurkat, HepG2, SKBR 3, Jurkat and Raji cell lysates. Flow Cyt: Jurkat cells.
This product was previously labelled as HDJ2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
解離定数（KD 値）KD = 4.20 x 10 -11 M Learn more about KD
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
精製度Tissue culture supernatant
KO cell lysates
Our Abpromise guarantee covers the use of ab126774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
機能Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.
配列類似性Contains 1 CR-type zinc finger.
Contains 1 J domain.
- Information by UniProt
- DJ 2 antibody
- DJ2 antibody
- DjA1 antibody
All lanes : Anti-DNAJA1 antibody [EPR7248] (ab126774) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DNAJA1 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Lanes 1-4: Merged signal (red and green). Green - ab126774 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa.
ab126774 Anti-DNAJA1 antibody [EPR7248] was shown to specifically react with DNAJA1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266437 (knockout cell lysate ab257925) was used. Wild-type and DNAJA1 knockout samples were subjected to SDS-PAGE. ab126774 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Lane 1: Wild-type DNAJA1 whole cell lysate (20 µg)
Lane 2: DNAJA1 knockout DNAJA1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: Jurkat whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab126774 observed at 45 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab126774 was shown to recognize DNAJA1 in wild-type HAP1 samples along with additional cross-reactive bands. No band was observed when DNAJA1 knockout samples were examined. Wild-type and DNAJA1 knockout samples were subjected to SDS-PAGE. Ab126774 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
ab126774 at 1/10 dilution staining DNAJA1 in permeabilized Jurkat cells by Flow cytometry (red). Rabbit IgG negative control (green).
All lanes : Anti-DNAJA1 antibody [EPR7248] (ab126774) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : SKBR 3 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab126774 は 2 報の論文で使用されています。
- Wang C et al. Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea. J Biol Chem 294:9873-9887 (2019). PubMed: 31076507
- Fiore D et al. N6-Isopentenyladenosine Inhibits Colorectal Cancer and Improves Sensitivity to 5-Fluorouracil-Targeting FBXW7 Tumor Suppressor. Cancers (Basel) 11:N/A (2019). PubMed: 31569395