Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Aorta)
Specification
Human Aorta
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase-K, Dako
Permeabilization
No
Blocking step
Protein Block (DAKO) as blocking agent for 20 minute(s) · Concentration: 100%
Other product details
Dilution
1/350
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Antibody Diluent
Secondary antibody
Name
Non-Abcam antibody was used: Dako Goat anti Rabbit Biotinylated
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/800
Additional data
Additional Notes
Staining worked with both Heat mediated eptiope retrieval (Dako Target Retrieval Solution) as well as Enzymatic retrieval (Proteinase-K 10 minutes @ 37c)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 02 2012