Anti-DMC1 抗体 [2H12/4] (ab11054)
Key features and details
- Mouse monoclonal [2H12/4] to DMC1
- Suitable for: IP, WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-DMC1 antibody [2H12/4]
DMC1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2H12/4] to DMC1 -
由来種
Mouse -
特異性
Specific for DMC1 - does not cross-react with the related protein Rad51. The antibody was tested on mouse meiotic chromosomes. It allowed localisation of the Dmc1 protein as numerous foci in the Leptotene and Zygotene stages of meiotic prophase, and fewer foci in the early Pachytene stage of meiotic prophase. -
アプリケーション
適用あり: IP, WB, IHC-P, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Cow, Human -
免疫原
Recombinant fusion protein (Human).
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ポジティブ・コントロール
- ICC:mouse meiotic chromosomes
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
2H12/4 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11054の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
ICC/IF | (1) |
Use at an assay dependent concentration.
Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight. |
特記事項 |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight. |
ターゲット情報
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機能
May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks. -
配列類似性
Belongs to the RecA family. DMC1 subfamily.
Contains 1 HhH domain. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 618398 Cow
- Entrez Gene: 11144 Human
- Entrez Gene: 13404 Mouse
- Entrez Gene: 362960 Rat
- Omim: 602721 Human
- SwissProt: Q14565 Human
- SwissProt: Q61880 Mouse
- Unigene: 339396 Human
see all -
別名
- Disrupted meiotic cDNA 1 homolog antibody
- Disrupted meiotic cDNA 1, yeast, homolog of antibody
- dJ199H16.1 antibody
see all
画像
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Overlay histogram showing HeLa cells stained with ab11054 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11054, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (24)
ab11054 は 24 報の論文で使用されています。
- Wang M et al. Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis. Theranostics 11:5010-5027 (2021). PubMed: 33754041
- Chen Y et al. The SUN1-SPDYA interaction plays an essential role in meiosis prophase I. Nat Commun 12:3176 (2021). PubMed: 34039995
- Ghouil R et al. BRCA2 binding through a cryptic repeated motif to HSF2BP oligomers does not impact meiotic recombination. Nat Commun 12:4605 (2021). PubMed: 34326328
- Hinch AG et al. The Configuration of RPA, RAD51, and DMC1 Binding in Meiosis Reveals the Nature of Critical Recombination Intermediates. Mol Cell 79:689-701.e10 (2020). PubMed: 32610038
- Hutka M et al. Exogenous Gonadotrophin Stimulation Induces Partial Maturation of Human Sertoli Cells in a Testicular Xenotransplantation Model for Fertility Preservation. J Clin Med 9:N/A (2020). PubMed: 31963729