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Anti-DLK-1 抗体 (ab21682)

Reviews (5)Q&A (6)References (27)
Western blot - Anti-DLK-1 antibody (ab21682)

    Key features and details

    • Rabbit polyclonal to DLK-1
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-DLK-1 antibody [EPR19830] (ab210471)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-DLK-1 antibody
      DLK-1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to DLK-1

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Mouse
      交差が予測される動物種: Rat, Cow, Human

    • 免疫原

      Synthetic peptide corresponding to Human DLK-1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab23442)

    • ポジティブ･コントロール

      • This antibody gave a positive signal in E18.5 Mouse Muscle Wild Type Tissue Lysate.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab21682の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/2000. Detects a band of approximately 45,48 kDa (predicted molecular weight: 41 kDa).

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.
    特記事項
    WB
    1/2000. Detects a band of approximately 45,48 kDa (predicted molecular weight: 41 kDa).

    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product.

    ターゲット情報

    • 機能

      May have a role in neuroendocrine differentiation.

    • 組織特異性

      Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.

    • 配列類似性

      Contains 6 EGF-like domains.

    • 翻訳後修飾

      N- and O-glycosylated.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • Adipocyte differentiation inhibitor protein antibody
      • Brevideltin antibody
      • Delta like 1 homolog antibody
      • Delta like homolog antibody
      • Delta like protein antibody
      • Delta1 antibody
      • DLK 1 antibody
      • DLK antibody
      • DLK-1 antibody
      • DLK1 antibody
      • DLK1_HUMAN antibody
      • FA 1 antibody
      • FA1 antibody
      • Fetal antigen 1 antibody
      • pG2 antibody
      • Preadipocyte factor 1 antibody
      • Pref 1 antibody
      • Pref antibody
      • Pref1 antibody
      • Protein delta homolog 1 antibody
      • Secredeltin antibody
      • ZOG antibody
      see all

    画像

    • Western blot - Anti-DLK-1 antibody (ab21682)
      Western blot - Anti-DLK-1 antibody (ab21682)
      All lanes : Anti-DLK-1 antibody (ab21682) at 1/2000 dilution ((5% Milk blocking))

      Lane 1 : E18.5 Mouse Muscle Wild Type Tissue Lysate
      Lane 2 : E18.5 Mouse Muscle DLK1 Knockout Tissue Lysate

      Lysates/proteins at 5 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 41 kDa
      Observed band size: 45,48 kDa why is the actual band size different from the predicted?
      Additional bands at: 15 kDa (possible cleavage fragment), 55 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 3 minutes


      This blot was produced using an 8% Tris-glycine gel under the Tris-glycine buffer system. The gel was run at 120V for 1 hour 15 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab21682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

       

      Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    参考文献 (27)

    ab21682 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab21682 は 27 報の論文で使用されています。

    • Araujo AN  et al. Comprehensive Assessment of Copy Number Alterations Uncovers Recurrent AIFM3 and DLK1 Copy Gain in Medullary Thyroid Carcinoma. Cancers (Basel) 13:N/A (2021). PubMed: 33435319
    • Eze UC  et al. Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia. Nat Neurosci 24:584-594 (2021). PubMed: 33723434
    • Dokoshi T  et al. Hyaluronan Degradation by Cemip Regulates Host Defense against Staphylococcus aureus Skin Infection. Cell Rep 30:61-68.e4 (2020). PubMed: 31914398
    • Grassi ES  et al. Hypoxia-induced release, nuclear translocation, and signaling activity of a DLK1 intracellular fragment in glioma. Oncogene 39:4028-4044 (2020). PubMed: 32205867
    • Jurek S  et al. Optimizing adipogenic transdifferentiation of bovine mesenchymal stem cells: a prominent role of ascorbic acid in FABP4 induction. Adipocyte 9:35-50 (2020). PubMed: 31996081
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 11 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DLK-1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate, PH6
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 05 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-DLK-1 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Bone)
    Permeabilization
    No
    Specification
    Bone
    Blocking step
    Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 19 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DLK-1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (placenta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate, pH6, 100C, 20 min
    Permeabilization
    No
    Specification
    placenta
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 19 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-DLK-1 antibody

     Poor
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (neurons)
    Permeabilization
    Yes - 0.2% Tween 20
    Specification
    neurons
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 23 2014

    Question

    Le adjunto el formulario relleno, me ha dado la impresion que es un
    formulario para cuando la inmunocitoquimica no sale, pero ya le digo
    que en mi caso es a la inversa, no deberia salir la inmuno, pq no esta
    esa proteina es mis celulas, y da la casualidad que si sale!
    Espero su respuesta!

    Read More
    Answer

    Muchas gracias por tu paciencia.

    Lamento la espera. Mis compañeros del laboratorio me han confirmado que este anticuerpo solo lo han testado en células MES en inmunocitoquímica, y en WB en lisados de MEL-1, F9 y Mouse EG.

    Incluso en nuestra imagen en la datasheet parece haber cierta tinción filamentosa. Estamos investigando el caso, y haremos las pruebas adicionales pertinentes.

    Tenemos otros dos anticuerpos anti DLK monoclonales, testados en ICC en células humanas:

    https://www.abcam.com/DLK-antibody-3A10-ab119930.html

    https://www.abcam.com/DLK-antibody-MM0514-9D8-ab89908.html]

    Si quisieras recibir alguno de ellos en compensación por las molestias de forma gratuita, no dudes en comunicármelo. Alternativamente, y dado que ab21682 está cubierto por la garantía Abpromise, si lo prefieres podemos directamente reembolsarte el importe del mismo, mandarte un anticuerpo contra otra proteína, o emitir un vale sin caducidad para futuras compras con nosotros.

    De nuevo te pido disculpas, y quedo a la espera de tu respuesta. ¡Que pases un buen día!

    Read More

    Question

    send testing discount code for testing in flow on human cells.

    Read More
    Answer

    x
    Je suis ravie que vous souhaitiez participer a notre offre et tester ab21682 en cytométrie de flux. Le code de réduction correspondant est xx . Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre anticorps primaire.
    Vous devez dans un premier temps soumettre une Abreview avec vos résultats en cytométrie de flux en notant ce code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de ab21682.
    Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches.
    Termes et Conditions : www.abcam.com/collaborationdiscount.

    Read More

    Question

    First issue: thank you for the offer: we would indeed appreciate a lot to receive a new vial of ab110363 and a purification kit for tissue culture supernatant ab109207 to replace the lost material. This could help us a lot.
    Second issue: Questionnaire for ab21682:
    IHC Questionnaire
    It will take approximately 5 minutes to complete the questionnaire.
    Please fill-in all fields so that we can better assist you
    1) Abcam product code ab21682, rabbit anti-DLK1

    2) Abcam order reference number or product batch number : GR31847-1
    3) Description of the problem: high aspecific background, impossible/difficult to pick up the DLK1 signal. Conclusion: antibody is not specific, but gives a very particular intracellular/perinuclear staining pattern (=golgi/ER?)
    4) Sample preparation:
    Species: rat pancreatic beta cells
    Type of sample: Fresh frozen sections, perfusion fixed frozen sections, PFA/formalin fixed paraffin embedded sections, cells in culture, other:
    Sample preparation : fixation in formol
    Positive control: unstimulated cells (MIX1), negative for DLK1
    Negative control : cells treated with specific compound to trigger DLK1 expression (MIX2), confirmed at level of qPCR (80.000 fold increase at RNA level), ICC with 5 other rabbit anti DLK1 antibodies, including ab110636
    5) Fixation step
    Yes/No
    If yes: Fixative agent and concentration : formaldehyde 3.7% in PBS
    Fixation time: 30 minutes to 1 day
    Fixation temperature: cold room 4°C
    6) Antigen retrieval method: none, not required for the other tested antibodies, which all were very specific (see picture)
    7) Permeabilization method:
    Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers?
    Permeabilizing agent and concentration:
    TritonX100, 0.5% in PBS
    8) Blocking agent (eg BSA, serum…): BSA 3%
    Concentration
    Blocking time: 30 to 60 minutes
    Blocking temperature room temperature
    9) Endogenous peroxidases blocked? No, not relevant
    Endogenous biotins blocked? No, not relevant
    10) Primary antibody (If more than one was used, describe in “additional notes”) :
    Concentration or dilution 1/250, 1/1000
    Diluent buffer: PBS
    Incubation time: overnight, 4°C
    11) Secondary antibody:
    Species: donkey anti rabbit
    Reacts against:
    Concentration or dilution: 1/3000
    Diluent buffer PBS
    Incubation time: 30 minutes
    Fluorochrome or enzyme conjugate: 488
    12) Washing after primary and secondary antibodies:
    Buffer: PBS+3% BSA
    Number of washes : 3x 5 minutes
    13) Detection method: 488 fluorescence microscope – DAPI for nuclei
    14) How many times have you run this staining? 3x, in parallel with other DLK1 antibodies (ab110636, santacruz and an non commercial ab)
    Do you obtain the same results every time? yes
    What steps have you altered to try and optimize the use of this antibody? Not, other antibodies have been used as possitve control, we have no need to optimize this ab further.
    Document attachment: see enclosed picture

    Read More
    Answer

    Thank you for these precisions.
    We are still working on your complaint for ab21682 (anti-DLK1) and we will come back to you as soon as possible.
    As requested, I have issued a free of charge replacement with the order number *******.
    To check the status of the order please contact our Customer Service team and reference this number.
    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    Read More

    Question

    Dear,
    We have a number of concerns:
    1) We were recently adviced to buy the antibody purification kit (ab102784) to remove azide from the monoclonal ab110636 (see letter below), which we need to be able to use in cell culture as indicated. In the mean time we have bought this kit together with a FITC-labelingskit and two new batches of DLK1 antibodies (ab110636 and ab21682). After clean up with ab102784, it was found that ab110636 did not bind to the adviced collums, infact the antibody eluted already with the first step, the bidingbuffer. Only a minor fraction could be recovered in the washing buffers and elution buffers. An overview of our concentration measurements is provided in the attached file. This finding was also confirmed by ICC wish failed to provide a signal on a positive cell sample.
    For ab21682 whitch was purified in parallel we did not notice this problem, but we only need this antibody as a negative control. It is Ab110636 which we should obtain in a purified, azide free form. What is possible?
    One of the goals was also to label the purified ab110636 with de labeling kit, which now cannot be used.
    2) ab21682 has been tested for ICC using 2 conditions, one negative and a second with a stimulated expression of DLK1, it was compared for its specificity to 5 other DLK1 antibodies (see attached figure). Our conclusion is that ab21682 is not specific for its target, but shows a high aspecific background staining, which is problematic since this antibody is mentioned in a few publications, and is as such the first choice to buy ffrom the website. It is however doubtfull whether this antibody can be used for ICC, and should not be sold for this purpose, since the "background" signal could be misinterpreted. I did not test it on paraffin samples for IHC
    Ab110636 on the contrary is highly specific.
    What could you propose?

    Read More
    Answer

    Thank you very much for your email and your patience.
    In regards to the first issue, with the purification of ab110363, I had contacted the laboratory. There is nothing special with this clone. The only thing which might could explain the result is, that the ab110636 you received is at a low concentration. Therefore it could be too dilute to bind and elute efficiently. I can therefore propose to send you a new vial of ab110363 and a purification kit for tissue culture supernatant ab109207 for free, so you can perform the experiment. Please let me know whether you would like to accept this offer.
    In regards to the ab21682 staining in ICC, I would be pleased to investigate this further. Can you please provide me with an image and the protocol details? I have attached a questionnaire which will help you to put the protocol details together. Once I have received this information, it will be easier for me to investigate and troubleshoot the results, as well as initiate internal actions if necessary. The specificity of the product is obviously very important. If the product is faulty, we will also replace it for you.
    Thank you very much for your cooperation. I am looking forward to hear back from you.

    Read More

    Question

    Original purchased 3 vials of ab21682 to use in an inhibition assay (bought in March 2012) but have since found that ab119930 is more efficient. Would like to switch out the unopened vials of ab21682.
    Neither antibody has been tested in inhibition assays.

    Read More
    Answer

    Thank you for calling Abcam last night.

    I have spoken to my colleagues about your wish to return the 2 vials of ab21682. Since they were purchased more than 2 weeks ago, we cannot accept them as a return, I am sorry about that. I understand that these antibodies are expensive, so below is a discount that will give you 30% off the next antibody that you purchase:

    Discount Code : *******
    Expiration Date: ***********

    If there is anything else I can help you with, please let me know.

    Read More

    Question

    Customer kindly called to discuss the possibility that ab21682 was recognizing an extracellular domain as they are seeing a neutralizing affect when using this product. They would like to know if we have excluded the possibility of this reaction and how this was performed. They are continuing their own research into this by testing alongside ab119930, which should react with the extracellular domain, however they do feel that this is something which we, the originator of this product, should look into as well.

    Read More
    Answer

    Thank you for contacting us regarding this possibility. I have contacted the lab regarding the possibility that this product is recognizing the extracellular domain. I’m afraid we have no testing data to show the specificity for the intracellular domain as we do not specifically test for this. We only have the fact that the immunogen is raised against the intracellular region and the peptide blocking on the WB. It could be possible that it may be cross reacting with the extracellular domain but it is just not something we currently test for. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

    Read More

    1-10 of 11 Abreviews or Q&A

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