Anti-Dkk3 抗体 (ab2459)
製品の概要
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製品名
Anti-Dkk3 antibody
Dkk3 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Dkk3 -
由来種
Goat -
特異性
This antibody is expected to recognise the 350 aa isoform only. -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
AAAALLGGEEI
, corresponding to C terminal amino acids 340-350 of 350 aa isoform DKK3. -
ポジティブ･コントロール
- 293 lysate.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.03 - 0.1 µg/ml. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 25029272 | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
組織特異性
Highest expression in heart, brain, and spinal cord. -
配列類似性
Belongs to the dickkopf family. -
ドメイン
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 27122 Human
- Omim: 605416 Human
- SwissProt: Q9UBP4 Human
- Unigene: 292156 Human
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別名
- Dickkopf 3 antibody
- Dickkopf 3 homolog (Xenopus laevis) antibody
- Dickkopf 3 homolog antibody
see all
画像
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Western blot - Anti-Dkk3 antibody (ab2459)Anti-Dkk3 antibody (ab2459) at 2 µg/ml + Human Cerebellum lysate at 35 µg -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dkk3 antibody (ab2459)This image is courtesy of an anonymous Abreviewab2459 staining DKK3 in human MDA-MB 436 cells by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C. The primary antibody was diluted 1/75 and incubated with the sample for 30 minutes at 22°C. A biotin conjugated rabbit polyclonal to goat Ig, diluted 1/200, was used as the secondary.
プロトコール
参考文献
This product has been referenced in:
- Loo L et al. Single-cell transcriptomic analysis of mouse neocortical development. Nat Commun 10:134 (2019). Read more (PubMed: 30635555) »
- Marthandan S et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . Read more (PubMed: 27140416) »
