Anti-Dkk3 抗体 (ab2459)
Key features and details
- Goat polyclonal to Dkk3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Dkk3 antibody
Dkk3 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Dkk3 -
由来種
Goat -
特異性
This antibody is expected to recognise the 350 aa isoform only. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
AAAALLGGEEI
, corresponding to C terminal amino acids 340-350 of 350 aa isoform DKK3.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.03 - 0.1 µg/ml. 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
組織特異性
Highest expression in heart, brain, and spinal cord. -
配列類似性
Belongs to the dickkopf family. -
ドメイン
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 27122 Human
- Omim: 605416 Human
- SwissProt: Q9UBP4 Human
- Unigene: 292156 Human
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別名
- Dickkopf 3 antibody
- Dickkopf 3 homolog (Xenopus laevis) antibody
- Dickkopf 3 homolog antibody
see all
画像
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Western blot - Anti-Dkk3 antibody (ab2459)Anti-Dkk3 antibody (ab2459) at 2 µg/ml + Human Cerebellum lysate at 35 µg
プロトコール
参考文献 (5)
ab2459 は 5 報の論文で使用されています。
- Loo L et al. Single-cell transcriptomic analysis of mouse neocortical development. Nat Commun 10:134 (2019). PubMed: 30635555
- Marthandan S et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . PubMed: 27140416
- Marthandan S et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . PubMed: 26339636
- Forsdahl S et al. Pax6 regulates the expression of Dkk3 in murine and human cell lines, and altered responses to Wnt signaling are shown in FlpIn-3T3 cells stably expressing either the Pax6 or the Pax6(5a) isoform. PLoS One 9:e102559 (2014). ICC/IF, WB ; Human, Mouse . PubMed: 25029272
- Saeb-Parsy K et al. MT1-MMP regulates urothelial cell invasion via transcriptional regulation of Dickkopf-3. Br J Cancer 99:663-9 (2008). WB ; Human . PubMed: 18665176
