Anti-Dkk3 抗体 (ab2459)

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Western blot - Anti-Dkk3 antibody (ab2459)

    Key features and details

    • Goat polyclonal to Dkk3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Dkk3 antibody [EPR15611] (ab186409)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Dkk3 antibody
      Dkk3 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to Dkk3

    • 由来種

      Goat

    • 特異性

      This antibody is expected to recognise the 350 aa isoform only.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide:

      AAAALLGGEEI

      , corresponding to C terminal amino acids 340-350 of 350 aa isoform DKK3.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab2459の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (2)
    Use a concentration of 0.03 - 0.1 µg/ml.

    1 hour primary incubation is recommended for this product.
    特記事項
    WB
    Use a concentration of 0.03 - 0.1 µg/ml.

    1 hour primary incubation is recommended for this product.

    ターゲット情報

    • 機能

      Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

    • 組織特異性

      Highest expression in heart, brain, and spinal cord.

    • 配列類似性

      Belongs to the dickkopf family.

    • ドメイン

      The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

    • 翻訳後修飾

      N- and O-glycosylated.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Dickkopf 3 antibody
        • Dickkopf 3 homolog (Xenopus laevis) antibody
        • Dickkopf 3 homolog antibody
        • Dickkopf homolog 3 antibody
        • Dickkopf related protein 3 antibody
        • Dickkopf related protein 3 Precursor antibody
        • Dickkopf WNT signaling pathway inhibitor 3 antibody
        • Dickkopf, Xenopus, homolog of, 3 antibody
        • Dickkopf-3 antibody
        • Dickkopf-related protein 3 antibody
        • Dickkopf3 antibody
        • DKK 3 antibody
        • Dkk-3 antibody
        • DKK3 antibody
        • DKK3_HUMAN antibody
        • hDkk 3 antibody
        • hDkk-3 antibody
        • hDkk3 antibody
        • Regulated in glioma antibody
        • REIC antibody
        • RIG antibody
        • RIG-like 5-6 antibody
        • RIG-like 7-1 antibody
        see all

      画像

      • Western blot - Anti-Dkk3 antibody (ab2459)
        Western blot - Anti-Dkk3 antibody (ab2459)
        Anti-Dkk3 antibody (ab2459) at 2 µg/ml + Human Cerebellum lysate at 35 µg

      参考文献 (6)

      ab2459 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2459 は 6 報の論文で使用されています。

      • Loo L  et al. Single-cell transcriptomic analysis of mouse neocortical development. Nat Commun 10:134 (2019). PubMed: 30635555
      • Yang Y  et al. LINC00459 sponging miR-218 to elevate DKK3 inhibits proliferation and invasion in melanoma. Sci Rep 9:19139 (2019). PubMed: 31844121
      • Marthandan S  et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . PubMed: 27140416
      • Marthandan S  et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . PubMed: 26339636
      • Forsdahl S  et al. Pax6 regulates the expression of Dkk3 in murine and human cell lines, and altered responses to Wnt signaling are shown in FlpIn-3T3 cells stably expressing either the Pax6 or the Pax6(5a) isoform. PLoS One 9:e102559 (2014). ICC/IF, WB ; Human, Mouse . PubMed: 25029272
      • Saeb-Parsy K  et al. MT1-MMP regulates urothelial cell invasion via transcriptional regulation of Dickkopf-3. Br J Cancer 99:663-9 (2008). WB ; Human . PubMed: 18665176

      レビューと Q&A

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      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-Dkk3 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Human MDA-MB-436 Breast Cancer Cells)
      Loading amount
      3 µg
      Specification
      Human MDA-MB-436 Breast Cancer Cells
      Gel Running Conditions
      Reduced Denaturing (10%)
      Blocking step
      1xCasein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      投稿 Apr 27 2010

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dkk3 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Human MDA-MB 436 cells grown subcutaneously in mou)
      Specification
      Human MDA-MB 436 cells grown subcutaneously in mou
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Sodium Citrate-EDTA (pH6)
      Permeabilization
      No
      Blocking step
      casein as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      投稿 Oct 27 2009

      Western blot abreview for Anti-Dkk3 antibody

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - other (Cerebrospinal fluid (CSF))
      Loading amount
      3 µg
      Specification
      Cerebrospinal fluid (CSF)
      Gel Running Conditions
      Reduced Denaturing (12% SDS)
      Blocking step
      Milk as blocking agent for 17 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: + 4°C
      Read More

      Ms. Christiane Rewerts

      Verified customer

      投稿 Jun 04 2009

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