Key features and details
- Goat polyclonal to Dkk3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab2459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.03 - 0.1 µg/ml.
1 hour primary incubation is recommended for this product.
|ICC/IF||Use at an assay dependent concentration. PubMed: 25029272|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
機能Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
組織特異性Highest expression in heart, brain, and spinal cord.
配列類似性Belongs to the dickkopf family.
ドメインThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
翻訳後修飾N- and O-glycosylated.
- Information by UniProt
- Dickkopf 3 antibody
- Dickkopf 3 homolog (Xenopus laevis) antibody
- Dickkopf 3 homolog antibody
Anti-Dkk3 antibody (ab2459) at 2 µg/ml + Human Cerebellum lysate at 35 µg
ab2459 staining DKK3 in human MDA-MB 436 cells by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C. The primary antibody was diluted 1/75 and incubated with the sample for 30 minutes at 22°C. A biotin conjugated rabbit polyclonal to goat Ig, diluted 1/200, was used as the secondary.
ab2459 は 5 報の論文で使用されています。
- Loo L et al. Single-cell transcriptomic analysis of mouse neocortical development. Nat Commun 10:134 (2019). PubMed: 30635555
- Marthandan S et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . PubMed: 27140416
- Marthandan S et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . PubMed: 26339636
- Forsdahl S et al. Pax6 regulates the expression of Dkk3 in murine and human cell lines, and altered responses to Wnt signaling are shown in FlpIn-3T3 cells stably expressing either the Pax6 or the Pax6(5a) isoform. PLoS One 9:e102559 (2014). ICC/IF, WB ; Human, Mouse . PubMed: 25029272
- Saeb-Parsy K et al. MT1-MMP regulates urothelial cell invasion via transcriptional regulation of Dickkopf-3. Br J Cancer 99:663-9 (2008). WB ; Human . PubMed: 18665176