Anti-DKK1 抗体 [EPR4759] (ab109416)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4759] to DKK1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DKK1 antibody [EPR4759]
DKK1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4759] to DKK1 -
由来種
Rabbit -
特異性
Although our internal data shows that this antibody is not suitable to detect DKK1 in rats, this antibody has been referenced in PMID 29299122 and 25469240 to work with rat derived cells. Please contact technical@abcam.com for further information.
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アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HEK-293, Caco-2, U-2 OS, MOLT4, A549, PC-3, NCI-H460, DU145 and SK-OV-3 whole cell lysates IHC-P: Human placenta and liver tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4759 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109416の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
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IHC-P |
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. 1/250.00000 - 1/500.00000 |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa). |
IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. 1/250.00000 - 1/500.00000 |
ターゲット情報
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機能
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
組織特異性
Placenta. -
配列類似性
Belongs to the dickkopf family. -
ドメイン
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 22943 Human
- Omim: 605189 Human
- SwissProt: O94907 Human
- Unigene: 40499 Human
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別名
- Dickkopf 1 antibody
- Dickkopf 1 homolog antibody
- Dickkopf 1 like antibody
see all
画像
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All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : U-2 OS cell lysate
Lane 4 : MOLT4 cell lysate
Lane 5 : HEK-293 cell lysate
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 30-45 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-DKK1 antibody [EPR4759] staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling DKK1 with Purified ab109416 at 1:800 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
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All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/10000 dilution
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29-40 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST.
HEK-293 and 293T cell are weak expressed cell lines for DKK1 protein (PMID: 18502762, PMID: 15378020).
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Anti-DKK1 antibody [EPR4759] (ab109416) at 1/1000 dilution + His-Tagged human DKK1 (aa 32 to 266) recombinant protein, 100ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDaBlocking/Diluting buffer and concentration 5% NFDM /TBST
Exposure time: 5.5 seconds
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All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution (purified)
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : NCI-H460 (Human Large Cell Lung Cancer epithelial cell) whole cell lysates
Lane 4 : DU145 (Human prostate carcinoma epithelial cell) whole cell lysates
Lane 5 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDaBlocking and diluting buffer: 5% NFDM/TBST.
The smear bands detected by ab109416 are similar to those reported by the papers (PMID: 18546262, 28090290, 24008318), which should be due to the glycosylation of DKK1. -
Immunohistochemical analysis of paraffin-embedded Human placenta tissue using 1/100 unpurified ab109416.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (40)
ab109416 は 40 報の論文で使用されています。
- Qian HG et al. Long non‑coding RNA LINC00238 suppresses the malignant phenotype of liver cancer by sponging miR‑522. Mol Med Rep 25:N/A (2022). PubMed: 35014686
- Zheng Y et al. The RNA-binding protein PCBP1 represses lung adenocarcinoma progression by stabilizing DKK1 mRNA and subsequently downregulating β-catenin. J Transl Med 20:343 (2022). PubMed: 35907982
- Sun HF et al. Single-cell RNA sequencing reveals cellular and molecular reprograming landscape of gliomas and lung cancer brain metastases. Clin Transl Med 12:e1101 (2022). PubMed: 36336787
- Shailesh H et al. Protein arginine methyltransferase 5 (PRMT5) activates WNT/ß-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3. J Cell Mol Med 25:1583-1600 (2021). PubMed: 33462997
- Qi L et al. HomeoboxC6 promotes metastasis by orchestrating the DKK1/Wnt/ß-catenin axis in right-sided colon cancer. Cell Death Dis 12:337 (2021). PubMed: 33795652