Anti-DKC1/Dyskerin 抗体 [EPR10399] (ab156877)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10399] to DKC1/Dyskerin
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DKC1/Dyskerin antibody [EPR10399]
DKC1/Dyskerin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10399] to DKC1/Dyskerin -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human DKC1/Dyskerin aa 350-450. The exact sequence is proprietary.
Database link: O60832 -
ポジティブ・コントロール
- Recombinant Human DKC1/Dyskerin protein (ab152334) can be used as a positive control in WB. SW480 cell lysate, MCF-7 cell lysate, HeLa cell lysate, paraffin-embedded Human melanoma tissue, paraffin-embedded Human placenta tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10399 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab156877の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
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ICC/IF |
1/100 - 1/250.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa. |
ICC/IF
1/100 - 1/250. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ターゲット情報
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機能
Isoform 1: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Isoform 3: Promotes cell to cell and cell to substratum adhesion, increases the cell proliferation rate and leads to cytokeratin hyper-expression (when overexpressed in HeLa cells). -
組織特異性
Ubiquitously expressed. -
関連疾患
Defects in DKC1 are a cause of dyskeratosis congenita X-linked recessive (XDKC) [MIM:305000]. XDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
Defects in DKC1 are the cause of Hoyeraal-Hreidarsson syndrome (HHS) [MIM:300240]. HHS is a multisystem disorder affecting males and is characterized by aplastic anemia, immunodeficiency, microcephaly, cerebellar hypoplasia, and growth retardation. -
配列類似性
Belongs to the pseudouridine synthase TruB family.
Contains 1 PUA domain. -
細胞内局在
Cytoplasm and Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies. - Information by UniProt
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参照データベース
- Entrez Gene: 1736 Human
- Entrez Gene: 245474 Mouse
- Entrez Gene: 170944 Rat
- Omim: 300126 Human
- SwissProt: O60832 Human
- SwissProt: Q9ESX5 Mouse
- SwissProt: P40615 Rat
- Unigene: 4747 Human
see all -
別名
- CBF5 antibody
- CBF5 homolog antibody
- Cbf5p homolog antibody
see all
画像
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All lanes : Anti-DKC1/Dyskerin antibody [EPR10399] (ab156877) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 58 kDa -
Immunohistochemical analysis of paraffin embedded Human melanoma tissue labeling DKC1/Dyskerin with ab156877 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling DKC1/Dyskerin with ab156877 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunofluorescence analysis of HeLa cells labeling DKC1/Dyskerin with ab156877 antibody at a dilution of 1/100.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab156877 は 4 報の論文で使用されています。
- Wang LJ et al. Gene Expression-Based Predication of RNA Pseudouridine Modification in Tumor Microenvironment and Prognosis of Glioma Patients. Front Cell Dev Biol 9:727595 (2021). PubMed: 35118063
- Yang Z et al. LncRNA MEG3 inhibits non-small cell lung cancer via interaction with DKC1 protein. Oncol Lett 20:2183-2190 (2020). PubMed: 32782535
- Saez I et al. The E3 ubiquitin ligase UBR5 interacts with the H/ACA ribonucleoprotein complex and regulates ribosomal RNA biogenesis in embryonic stem cells. FEBS Lett N/A:N/A (2019). PubMed: 31365120
- Quan Wang J et al. Discovery of new chromen-4-one derivatives as telomerase inhibitors through regulating expression of dyskerin. J Enzyme Inhib Med Chem 33:1199-1211 (2018). PubMed: 30132373