ChIP abreview for Anti-dimethyl Arginine antibody [21C7] - ChIP Grade

Excellent
Abreviews
Application
ChIP
Sample
Human Cell lysate - nuclear (HL-60 cells)
Specification
HL-60 cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Taqman
Positive control
input DNA
Negative control
No antibody controls.

Other product details

Dilution
1/100
Incubation time
0 minute(s)

Additional data

PubMed ID
15964820: View
Additional Notes
The antibody mentioned in the paper (ab413 Pan dimethyl arginine) worked well in Chromatin Ip on the promoter of a retinoid regulated gene in human HL-60 cell line. We measured the methylation level by Taqman QPCR. The levels had big changes with low backgrounds.

According to these data arginine methylation of the histone H4 produced an increased retinoid responsiveness of the studied gene (TGM2).

Dr. Balint Laszlo Balint

Verified customer

投稿 Oct 04 2005

登録