製品の概要

  • 製品名

    Anti-dimethyl Arginine antibody [21C7]
  • 製品の詳細

    Mouse monoclonal [21C7] to dimethyl Arginine
  • 由来種

    Mouse
  • 特異性

    This antibody reacts with free and bound asymmetric NG-NG-dimethyl arginine. It is not known whether the antibody reacts with symmetric dimethyl arginine.
  • アプリケーション

    適用あり: IP, WB, ChIPmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Asymmetric NG -N G -dimethyl arginine (two methyl groups on one of the terminal nitrogen atoms of the guanidinium group)

  • 特記事項

    This antibody will be of use in chromatin and transcription factor research to monitor methylation status. Since it is specific to dimethyl arginine it can be used in parallel with ab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethyl arginine only) to monitor the exact modification status.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution.
WB 1/50 - 1/500.
ChIP Use at an assay dependent concentration. Use 5µl for 4x106 cells.

ターゲット情報

  • 別名

    • Di methyl Arginine antibody

参考文献

This product has been referenced in:

  • Albrecht LV  et al. Arginine methylation is required for canonical Wnt signaling and endolysosomal trafficking. Proc Natl Acad Sci U S A 115:E5317-E5325 (2018). Read more (PubMed: 29773710) »
  • Yaseen I  et al. Mycobacteria modulate host epigenetic machinery by Rv1988 methylation of a non-tail arginine of histone H3. Nat Commun 6:8922 (2015). Read more (PubMed: 26568365) »
See all 9 Publications for this product

レビューと Q&A

Filter by Application

Filter by Species

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Application
ChIP
Sample
Human Cell lysate - nuclear (HL-60 cells)
Specification
HL-60 cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Taqman
Positive control
input DNA
Negative control
No antibody controls.

Dr. Balint Laszlo Balint

Verified customer

投稿 Oct 04 2005

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