Anti-dimethyl Arginine 抗体 [21C7] (ab413)

Abreviews (1)Q&A (9)Specific References (7)

製品の概要

  • 製品名
    Anti-dimethyl Arginine antibody [21C7]
  • 製品の詳細
    Mouse monoclonal [21C7] to dimethyl Arginine
  • 由来種
    Mouse
  • 特異性
    This antibody reacts with free and bound asymmetric NG-NG-dimethyl arginine. It is not known whether the antibody reacts with symmetric dimethyl arginine.
  • アプリケーション
    適用あり: IP, WB, ChIPmore details
  • 免疫原

    Asymmetric NG -N G -dimethyl arginine (two methyl groups on one of the terminal nitrogen atoms of the guanidinium group)

  • 特記事項

    This antibody will be of use in chromatin and transcription factor research to monitor methylation status. Since it is specific to dimethyl arginine it can be used in parallel with ab412 (detects monomethyl and dimethyl arginine) and ab414 (detects monomethyl arginine only) to monitor the exact modification status.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution.
WB 1/50 - 1/500.
ChIP Use at an assay dependent concentration. Use 5µl for 4x106 cells.

ターゲット情報

  • 別名
    • Di methyl Arginine antibody

参考文献

This product has been referenced in:

See all 7 Publications for this product

ab413 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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Our customer wants to find antibodies specific for asymmetrical methyl arginine and specific for symmetirical antibody respectly. So please let me know this antibody can detect only asymmetrical methyl arginine or not. If you know specific antibody ple...

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Thank you for your enquiry.

I can confirm that ab412 mono and dimethyl Arginine antibody [7E6] cross reacts with both symetric and assymetric methyl arginine. However, the ab413dimethyl Arginineantibodyis specific to ADMA only.

ht...

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samples; HEK293 cells block: 5% milk ab413, ab415: black membrane, protein ladder in white Ab 1/1000 suggestion: use less primary and/or secondary antibody ab109451: lots of bands, Ab 1/1000 suggestion: use less primary ...

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Thank you for your phone call. I am glad to hear that for ab109451 the protocol tips did work. I am very sorry that unfortunately, for ab413 and ab415, the tips did not improve your results. However, the details provided enable us to closely monitor th...

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samples; HEK293 cells block: 5% milk ab413, ab415: black membrane, protein ladder in white Ab 1/1000 suggestion: use less primary and/or secondary antibody ab109451: lots of bands, Ab 1/1000 suggestion: use less primary antibody (recommended ...

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Thank you for your phone call. I am sorry to hear that these 3 antibodies are not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality....

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ChIP abreview for Anti-dimethyl Arginine antibody [21C7] - ChIP Grade

 Excellent
Abreviews
Application
ChIP
Sample
Human Cell lysate - nuclear (HL-60 cells)
Specification
HL-60 cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Taqman
Positive control
input DNA
Negative control
No antibody controls.
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Username

Dr. Balint Laszlo Balint

Verified customer

投稿 Oct 04 2005

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 100537 DESCRIPTION OF THE PROBLEM I ordered three antibodies, ab412, ab413, and ab414. I am currently trying to optimize the conditions for Western blotting using these antibodies. None...

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Thank you for your enquiry. Thank you also for completing our questionnaire so comprehensively. I am sorry that you have been having difficulties with our antisera against methyl arginine. I have examined your questionnaire and it seems as if you a...

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Concerning ab415, ab414, ab413 - says whole antiserum; is it tisue culture supernatant or ascites? Do ab412 and ab413 cross-reat with symmetrical Ng/Na - dimethyl arginine?

Thank you for your patience. Ab415, ab414, and ab413 are ascites. It has not been determinded as to whether ab412 and ab413 cross-react with symmetrical Ng/Na - dimethyl arginine.

I recently purchased an Ab 413, the anti-dimethyl Arginine antibody.  There is no information regarding concentration of this antibody in the product information sheet, and while I realize a specfic concentration may not be known or tested, I am wonder...

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Thank you for your enquiry and your interest in our products. We are sorry to inform you that there is no protein concentration for polyclonal antibodies that are not affinity purified. This antibody is provided as whole serum and the IgG concent...

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I am interested in setting up an ELISA to quantitate serum concentrations of asymmetric dimethylarginine. However, I am concerned about cross-reactivity with free symmetric dimethylarginine. Do you have updated information on the extent of cross r...

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This antibody has not been tested in ELISA. All the latest information that we have about this antibody is found on the datasheet.

Has this Ab been shown to react with diMeArg in a peptide when the surrounding sequence is very different from the sequence used to generate the Ab? If so, how many different sequences have been tested? Without information on the context dependency, ...

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The epitope is di-methyl arginine independent of the surrounding sequence and has been used on numerous different diMeArg peptides.

What peptide sequence was used as the antigen?

Sorry, but the immunogen of this antibody is confidential. All we can say is that it contained methylated arginine (the antibody is inhibited by the modified amino acid).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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