Anti-Digoxigenin 抗体 [21H8] (ab420)
Key features and details
- Mouse monoclonal [21H8] to Digoxigenin
- Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization, Southern Blot
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-Digoxigenin antibody [21H8]
Digoxigenin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [21H8] to Digoxigenin -
由来種
Mouse -
アプリケーション
適用あり: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization, Southern Blotmore details -
種交差性
交差種: Species independent -
免疫原
Other Immunogen Type corresponding to Digoxigenin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section. -
バッファー
Constituents: 1.2114% Tris, 0.7507% Glycine, 2% Sucrose -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
21H8 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab420の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/5000. ab419 can be used for the detection of a diverse range of digoxigenin labelled compounds and in a variety of techniques
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ELISA |
1/1000 - 1/10000.
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IHC-P |
1/200 - 1/2000.
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IHC-Fr |
1/200 - 1/2000.
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In situ hybridization |
Use at an assay dependent concentration.
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Southern Blot |
1/500 - 1/5000.
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特記事項 |
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WB
1/500 - 1/5000. ab419 can be used for the detection of a diverse range of digoxigenin labelled compounds and in a variety of techniques |
ELISA
1/1000 - 1/10000. |
IHC-P
1/200 - 1/2000. |
IHC-Fr
1/200 - 1/2000. |
In situ hybridization
Use at an assay dependent concentration. |
Southern Blot
1/500 - 1/5000. |
ターゲット情報
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関連性
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications. -
別名
- 1672-46-4 antibody
- BRN 0096479 antibody
- DIG antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab420 は 33 報の論文で使用されています。
- Cortes-Troncoso J et al. T cell exosome-derived miR-142-3p impairs glandular cell function in Sjögren's syndrome. JCI Insight 5:N/A (2020). PubMed: 32376798
- Christ W et al. Puumala and Andes Orthohantaviruses Cause Transient Protein Kinase R-Dependent Formation of Stress Granules. J Virol 94:N/A (2020). PubMed: 31723021
- Budzak J et al. Dynamic colocalization of 2 simultaneously active VSG expression sites within a single expression-site body in Trypanosoma brucei. Proc Natl Acad Sci U S A 116:16561-16570 (2019). PubMed: 31358644
- Mackness BC et al. Antibody Fc engineering for enhanced neonatal Fc receptor binding and prolonged circulation half-life. MAbs 11:1276-1288 (2019). PubMed: 31216930
- Lee KJ et al. Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer. PLoS One 14:e0223725 (2019). PubMed: 31596905