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Anti-Dhh 抗体 [EPR23386-212] (ab270453)

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Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)
  • Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dhh antibody [EPR23386-212] (ab270453)
  • Anti-Dhh antibody [EPR23386-212] (ab270453)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23386-212] to Dhh
  • Suitable for: WB, IHC-P
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-Dhh antibody [EPR23386-212]
    Dhh 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23386-212] to Dhh

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human testis and Human colon tissue lysates; Recombinant Human Desert Hedgehog/Dhh protein. IHC-P: Human testis tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23386-212

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC
Human
IHC-Fr
Mouse
Rat
IHC-P
Human
IP
Human
WB
Human
アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 44 kDa.
IHC-P
1/500.
特記事項
WB
1/1000. Predicted molecular weight: 44 kDa.
IHC-P
1/500.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • ターゲット情報

    • 機能

      Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.

    • 関連疾患

      Defects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD) [MIM:607080]. PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity.
      Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM) [MIM:233420]; also known as male-limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks.

    • 配列類似性

      Belongs to the hedgehog family.

    • 翻訳後修飾

      The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). This covalent modification appears to play an essential role in restricting the spatial distribution of the protein activity to the cell surface. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.

    • 細胞内局在

      Secreted > extracellular space. The C-terminal peptide diffuses from the cell and Cell membrane. The N-terminal peptide remains associated with the cell surface.
    • Information by UniProt

    • 参照データベース

      • 別名

        • C78960 antibody
        • Desert hedgehog (Drosophila) homolog antibody
        • Desert hedgehog antibody
        • Desert hedgehog homolog (Drosophila) antibody
        • Desert hedgehog protein C-product antibody
        • Desert hedgehog protein precursor antibody
        • DHH antibody
        • DHH_HUMAN antibody
        • GDXYM antibody
        • HHG-3 antibody
        • Hira antibody
        • SRXY7 antibody
        see all

      画像

      • Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)
        Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)
        Anti-Dhh antibody [EPR23386-212] (ab270453) at 1/1000 dilution + Recombinant Human Desert Hedgehog/Dhh protein (aa 23-198) 20ng

        Secondary
        Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

        Predicted band size: 44 kDa
        Observed band size: 19 kDa why is the actual band size different from the predicted?



        Blocking and diluting buffer and concentration: 5% NFDM/TBST   

        Exposure time: 10 seconds

      • Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)
        Western blot - Anti-Dhh antibody [EPR23386-212] (ab270453)
        All lanes : Anti-Dhh antibody [EPR23386-212] (ab270453) at 1/1000 dilution

        Lane 1 : Human testis tissue lysate
        Lane 2 : Human colon tissue lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

        Predicted band size: 44 kDa
        Observed band size: 19 kDa why is the actual band size different from the predicted?



        Blocking and diluting buffer and concentration: 5% NFDM/TBST

        The molecular weight observed is consistent with what has been described in the literature.  Band at 19kDa could be DHH N-terminal fragment (PMID: 31018998).  

        The expression profile observed is consistent with what has been described in the literature(PMID:22132805).  

        Exposure time: 3 minutes

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dhh antibody [EPR23386-212] (ab270453)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dhh antibody [EPR23386-212] (ab270453)

        Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Dhh with ab270453 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human testis (PMID: 22132805). The section was incubated with ab270453 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      • Anti-Dhh antibody [EPR23386-212] (ab270453)
        Anti-Dhh antibody [EPR23386-212] (ab270453)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab270453 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab270453 は論文での使用が確認できていません。

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