Anti-DFNA5/GSDME 抗体 [EPR19859-60] - C-terminal (ab221843)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19859-60] to DFNA5/GSDME - C-terminal
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal
DFNA5/GSDME 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19859-60] to DFNA5/GSDME - C-terminal -
由来種
Rabbit -
特異性
Expression levels of the target protein vary with sample type and some optimisation may be required.
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アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate. HEK-293 transfected with a myc tagged, DFNA5/GSDME (C-terminus) expression vector, whole cell lysate. Human fetal brain lysate. IP: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19859-60 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab221843の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 36, 55 kDa (predicted molecular weight: 54 kDa).
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IP |
1/40.
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特記事項 |
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WB
1/1000. Detects a band of approximately 36, 55 kDa (predicted molecular weight: 54 kDa). |
IP
1/40. |
ターゲット情報
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組織特異性
Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta. -
関連疾患
Defects in DFNA5 are the cause of deafness autosomal dominant type 5 (DFNA5) [MIM:600994]. DFNA5 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. -
配列類似性
Belongs to the gasdermin family. - Information by UniProt
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参照データベース
- Entrez Gene: 1687 Human
- Omim: 608798 Human
- SwissProt: O60443 Human
- Unigene: 520708 Human
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別名
- 2310037D07Rik antibody
- 4932441K13Rik antibody
- Deafness, autosomal dominant 5 antibody
see all
画像
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All lanes : Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/5000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 2 : DFNA5/GSDME knockout SH-SY5Y, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Exposure time: 30 secondsBlocking/Dilution: 5% NFDM/TBST.
The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.
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All lanes : Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/1000 dilution
Lane 1 : HEK-293 (human embryonic kidney epithelial cell) transfected with a control vector containing DDDDK tag, whole cell lysate
Lane 2 : HEK-293 transfected with a DDDDK tagged, DFNA5/GSDME (N-terminus) expression vector, whole cell lysate
Lane 3 : HEK-293 transfected with a myc tagged, DFNA5/GSDME (C-terminus) expression vector, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 5 secondsBlocking/Dilution: 5% NFDM/TBST .
This antibody is specific to the C-terminus of DFNA5/GSDME.
The cell lysates were kindly provided by our collaborator, Dr. Feng Shao, NIBS.
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Anti-DFNA5/GSDME antibody [EPR19859-60] - C-terminal (ab221843) at 1/1000 dilution + human fetal brain at 10 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 3 minutesBlocking/Dilution: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 28459430).
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DFNA5/GSDME was immunoprecipitated from 0.35 mg of SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab221843 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab221843 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: SH-SY5Y whole cell lysate 10ug (Input).
Lane 2: ab221843 IP in SH-SY5Y whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221843 in SH-SY5Y whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Observed MW 55 kDa.
Exposure time: 1 second.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab221843 は 1 報の論文で使用されています。
- Wang Y et al. Chemotherapy drugs induce pyroptosis through caspase-3 cleavage of a Gasdermin. Nature N/A:N/A (2017). PubMed: 28459430