Anti-Detyrosinated alpha Tubulin 抗体 - Microtubule Marker (ab48389)
Key features and details
- Rabbit polyclonal to Detyrosinated alpha Tubulin - Microtubule Marker
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker
Detyrosinated alpha Tubulin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Detyrosinated alpha Tubulin - Microtubule Marker -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
A 10 residue synthetic peptide of the C terminal domain of human alpha Tubulin.
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特記事項
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 10/11/2017. The following lots are still unpurified and still in stock as of 10/11/2017- GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6, GR3181389-7. Lot numbers other than GR315444-5, GR315444-3 GR285350-3, GR3181389-3, GR3181389-4, GR3181389-5, GR3181389-6, GR3181389-7 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab48389の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (6) |
1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).
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特記事項 |
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WB
1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa). |
ターゲット情報
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関連性
Microtubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. The building block of a microtubule is the tubulin subunit, a heterodimer of alpha and beta tubulin. Both of these monomers are found in all eukaryotes, and their sequences are highly conserved. The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. This antibody recognizes detyrosinated alpha tubulin, a post translational modification of alpha tubulin. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 7277 Human
- Entrez Gene: 7278 Human
- Omim: 191110 Human
- Omim: 602528 Human
- SwissProt: P68366 Human
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別名
- Alpha tubulin 1 antibody
- TUBA1 antibody
- TUBA2 antibody
see all
画像
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All lanes : Anti-Detyrosinated alpha Tubulin antibody - Microtubule Marker (ab48389) at 1/500 dilution
Lane 1 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Human Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-3 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Lane 4 : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Detyrosinated alpha Tubulin contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (77)
ab48389 は 77 報の論文で使用されています。
- Rafiq NM et al. JIP3 links lysosome transport to regulation of multiple components of the axonal cytoskeleton. Commun Biol 5:5 (2022). PubMed: 35013510
- Moriarty RA et al. RNA localization in confined cells depends on cellular mechanical activity and contributes to confined migration. iScience 25:103845 (2022). PubMed: 35198898
- Halpern AR et al. Versatile, do-it-yourself, low-cost spinning disk confocal microscope. Biomed Opt Express 13:1102-1120 (2022). PubMed: 35284165
- Tavares S et al. FER regulates endosomal recycling and is a predictor for adjuvant taxane benefit in breast cancer. Cell Rep 39:110584 (2022). PubMed: 35385742
- Phyo SA et al. Transcriptional, Post-Transcriptional, and Post-Translational Mechanisms Rewrite the Tubulin Code During Cardiac Hypertrophy and Failure. Front Cell Dev Biol 10:837486 (2022). PubMed: 35433678