Anti-Desmoplakin I+II 抗体 [DP2.15] (ab16434)
製品の概要
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製品名Anti-Desmoplakin I+II antibody [DP2.15]
Desmoplakin I+II 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [DP2.15] to Desmoplakin I+II
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由来種Mouse
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アプリケーション適用あり: ICC, ICC/IF, WB, IHC-Frmore details
適用なし: IHC-P -
種交差性交差種: Mouse, Rat, Chicken, Cow, Human
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免疫原
Full length protein (Cow).
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ポジティブ･コントロール
- Positive cell lines: Human A432 and MCF-7; Rat MH1C1; Bovine MDBK and BMGE. Stratified epithelia, simple epithelia, including glands, urothelium, thymic reticular epithelium, hepatocytes, intercalated disks of myocardium and arachnoid cells of meninges.
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特記事項Suitable for use in the detection of primary and metastatic carcinomas.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.1% Sodium Azide
Constituents: 0.5% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノモノクローナル
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クローン名DP2.15
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab16434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | 1/200. | |
| ICC/IF | 1/100. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 331 kDa. | |
| IHC-Fr | 1/10. |
追加情報
Is unsuitable for IHC-P.
ターゲット情報
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機能Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.
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組織特異性Isoform DPI is apparently an obligate constituent of all desmosomes. Isoform DPII resides predominantly in tissues and cells of stratified origin.
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関連疾患Keratoderma, palmoplantar, striate 2
Cardiomyopathy, dilated, with woolly hair and keratoderma
Arrhythmogenic right ventricular dysplasia, familial, 8
Skin fragility-woolly hair syndrome
Epidermolysis bullosa, lethal acantholytic
Cardiomyopathy, dilated, with woolly hair, keratoderma, and tooth agenesis -
配列類似性Belongs to the plakin or cytolinker family.
Contains 17 plectin repeats.
Contains 1 SH3 domain.
Contains 6 spectrin repeats. -
ドメインIts association with epidermal and simple keratins is dependent on the tertiary structure induced by heterodimerization of these intermediate filaments proteins and most likely involves recognition sites located in the rod domain of these keratins.
The N-terminal region is required for localization to the desmosomal plaque and interacts with the N-terminal region of plakophilin 1.
The three tandem plakin repeat regions in the C-terminus mediate binding to intermediate filaments. -
翻訳後修飾Ser-2849 is probably phosphorylated by a cAMP-dependent protein kinase. Phosphorylation on Ser-2849 probably affects its association with epidermal, simple cytokeratins and VIM intermediate filaments.
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other desmoplakin molecules, to other proteins such as keratin, envoplakin, periplakin and involucrin, and to lipids like omega-hydroxyceramide (PubMed:9651377). -
細胞内局在Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Innermost portion of the desmosomal plaque. Colocalizes with epidermal KRT5-KRT14 and simple KRT8-KRT18 keratins and VIM intermediate filaments network (PubMed:12802069). Localizes at the intercalated disk in cardiomyocytes (By similarity).
- Information by UniProt
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参照データベース
- Entrez Gene: 420869 Chicken
- Entrez Gene: 514360 Cow
- Entrez Gene: 1832 Human
- Entrez Gene: 109620 Mouse
- Entrez Gene: 306871 Rat
- Omim: 125647 Human
- SwissProt: P15924 Human
- SwissProt: E9Q557 Mouse
see all -
別名
- 250/210 kDa paraneoplastic pemphigus antigen antibody
- DCWHKTA antibody
- Desmoplakin (DPI DPII) antibody
see all
画像
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![Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin I+II antibody [DP2.15] (ab16434)](https://www.abcam.co.jp/ps/products/16/ab16434/Images/ab14434_1 copy.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin I+II antibody [DP2.15] (ab16434)This image is courtesy of an anonymous Abreviewab16434 staining A431 cells by ICC/IF. Cells were methanol fixed and blocked in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/100 (PBS+10%FBS) and incubated with the sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/1500, was used as the secondary. -
![Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin I+II antibody [DP2.15] (ab16434)](https://www.abcam.co.jp/ps/products/16/ab16434/Images/ab16434-233955-ab16434iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Desmoplakin I+II antibody [DP2.15] (ab16434)This image is courtesy of an anonymous Abreviewab16434 staining Desmoplakin I+II in rat glial cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 5% serum) for 2 hours at 25°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.
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![Western blot - Anti-Desmoplakin I+II antibody [DP2.15] (ab16434)](https://www.abcam.co.jp/ps/products/16/ab16434/Images/ab16434-305988-16434.jpg)
Western blot - Anti-Desmoplakin I+II antibody [DP2.15] (ab16434)Image courtesy of an anonymous AbReviewAll lanes : Anti-Desmoplakin I+II antibody [DP2.15] (ab16434) at 1/500 dilution
Lane 1 : Control MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) treated with Desmoplakin I+II RNAi, cell lysate
Lysates/proteins at 40 µg per lane.
Developed using the ECL technique.
Predicted band size: 331 kDa
プロトコール
参考文献
This product has been referenced in:
- Clabault H et al. Effects of selective serotonin-reuptake inhibitors (SSRIs) on human villous trophoblasts syncytialization. Toxicol Appl Pharmacol 349:8-20 (2018). WB . Read more (PubMed: 29679653) »
- Kolahi K et al. Real-Time Tracking of BODIPY-C12 Long-Chain Fatty Acid in Human Term Placenta Reveals Unique Lipid Dynamics in Cytotrophoblast Cells. PLoS One 11:e0153522 (2016). Read more (PubMed: 27124483) »
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