Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7420] to Desmoglein 3/PVA - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
製品名Anti-Desmoglein 3/PVA antibody [EPR7420] - BSA and Azide free
Desmoglein 3/PVA 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7420] to Desmoglein 3/PVA - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC,ICC/IF,IHC-P or IP
Synthetic peptide within Human Desmoglein 3/PVA aa 900-1000. The exact sequence is proprietary.
ab248226 is the carrier-free version of ab128927 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248226 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 108 kDa).|
機能Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
組織特異性Epidermis, tongue, tonsil, esophagus and carcinomas.
配列類似性Contains 4 cadherin domains.
ドメインThree calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
細胞内局在Cell membrane. Cell junction > desmosome.
- Information by UniProt
- 130 kD pemphigus vulgaris antigen antibody
- 130 kDa pemphigus vulgaris antigen antibody
- Balding antibody
All lanes : Anti-Desmoglein 3/PVA antibody [EPR7420] (ab128927) at 1/1000 dilution
Lane 1 : Human tonsil lysate
Lane 2 : A431 lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 108 kDa
This data was developed using ab128927, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.