Anti-Desmoglein 1/DSG1 抗体 [32-2B] (ab108490)
Key features and details
- Mouse monoclonal [32-2B] to Desmoglein 1/DSG1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
製品の概要
-
製品名
Anti-Desmoglein 1/DSG1 antibody [32-2B]
Desmoglein 1/DSG1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [32-2B] to Desmoglein 1/DSG1 -
由来種
Mouse -
特異性
This antibody was tested for its ability to stain epithelia and tumours. 32-2B is a reliable epithelial marker that may have a useful role in diagnostic histopathology. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Full length protein corresponding to Desmoglein 1/DSG1.
-
ポジティブ・コントロール
- This antibody gave a positive result in IHC in the following FFPE tissue: Human skin melanoma.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
-
精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
32-2B -
ミエローマ
NS1 -
アイソタイプ
IgG2a -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108490の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
特記事項 |
---|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
組織特異性
Epidermis, tongue, tonsil and esophagus. -
関連疾患
Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger. -
配列類似性
Contains 4 cadherin domains. -
ドメイン
Calcium may be bound by the cadherin-like repeats. -
細胞内局在
Cell membrane. Cell junction > desmosome. - Information by UniProt
-
参照データベース
- Entrez Gene: 1828 Human
- Omim: 125670 Human
- SwissProt: Q02413 Human
- Unigene: 2633 Human
-
別名
- Cadherin family member 4 antibody
- CDHF4 antibody
- Desmoglein-1 antibody
see all
画像
-
IHC image of Desmoglein 1/DSG1 staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab108490, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab108490 は 2 報の論文で使用されています。
- Conn IG et al. Immunohistochemical staining with monoclonal antibody 32-2B to desmosomal glycoprotein 1. Its role in the histological assessment of urothelial carcinomas. Br J Urol 65:176-80 (1990). PubMed: 2317652
- Vilela MJ et al. Monoclonal antibody to desmosomal glycoprotein 1--a new epithelial marker for diagnostic pathology. J Pathol 153:365-75 (1987). PubMed: 3323436