Anti-Desmoglein 1/DSG1 抗体 [27B2] (ab12077)
![Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)](https://www.abcam.co.jp/ps/products/12/ab12077/Images/ab12077-336311-anti-desmoglein-1-antibody-27b2-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)")
Key features and details
- Mouse monoclonal [27B2] to Desmoglein 1/DSG1
- Suitable for: IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Desmoglein 1/DSG1 antibody [27B2]
Desmoglein 1/DSG1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [27B2] to Desmoglein 1/DSG1 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Frmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-Fr: Human normal skin.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
27B2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12077の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | (1) |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
|
IHC-Fr
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
組織特異性
Epidermis, tongue, tonsil and esophagus. -
関連疾患
Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger. -
配列類似性
Contains 4 cadherin domains. -
ドメイン
Calcium may be bound by the cadherin-like repeats. -
細胞内局在
Cell membrane. Cell junction > desmosome. - Information by UniProt
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参照データベース
- Entrez Gene: 1828 Human
- Entrez Gene: 13510 Mouse
- Omim: 125670 Human
- SwissProt: Q02413 Human
- SwissProt: Q61495 Mouse
- Unigene: 2633 Human
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別名
- Cadherin family member 4 antibody
- CDHF4 antibody
- Desmoglein-1 antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)
IHC image of ab12077 staining in 100% methanol fixed frozen tissue section of human skin.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab12077 (1μg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab12077.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
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![Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)](https://www.abcam.co.jp/ps/products/12/ab12077/Images/ab12077-19418-anti-desmoglein-1-antibody-27b2-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] (ab12077)This image is courtesy of an anonymous Abreview.ab12077 staining Desmoglein 1/DSG1 in human skin tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, permeabilized using 0.4% Triton X-100, blocked with 10% serum for 30 minutes at 25°C, then incubated with ab12077 at 5µg/ml for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-mouse polyclonal (highly adsorbed), used at a 1/1000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab12077 は 5 報の論文で使用されています。
- Simpson CL et al. NIX initiates mitochondrial fragmentation via DRP1 to drive epidermal differentiation. Cell Rep 34:108689 (2021). PubMed: 33535046
- Yuan J et al. SHROOM2 inhibits tumor metastasis through RhoA-ROCK pathway-dependent and -independent mechanisms in nasopharyngeal carcinoma. Cell Death Dis 10:58 (2019). PubMed: 30683844
- Tsutsumi M et al. Mechanical-stimulation-evoked calcium waves in proliferating and differentiated human keratinocytes. Cell Tissue Res 338:99-106 (2009). IHC-Fr ; Human . PubMed: 19657674
- Zhang X & Tseng H Basonuclin-null mutation impairs homeostasis and wound repair in mouse corneal epithelium. PLoS ONE 2:e1087 (2007). IHC-Fr ; Mouse . PubMed: 17971852
- Wu H et al. Desmoglein isotype expression in the hair follicle and its cysts correlates with type of keratinization and degree of differentiation. J Invest Dermatol 120:1052-7 (2003). PubMed: 12787134