Anti-Desmocollin 3 抗体 [EPR7486] (ab126768)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7486] to Desmocollin 3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Desmocollin 3 antibody [EPR7486]
Desmocollin 3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7486] to Desmocollin 3 -
由来種
Rabbit -
特異性
This antibody cross-reacts weakly with Desmocollin 2.
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アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- A431, Human skin, and Human espophagus lysates; and A431 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 2.40 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7486 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab126768の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 100 kDa.
|
特記事項 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 100 kDa. |
ターゲット情報
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機能
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. -
組織特異性
Epidermis, buccal mucosa, esophagus and cervix. -
関連疾患
Defects in DSC3 are the cause of hypotrichosis and recurrent skin vesicles (HRSV) [MIM:613102]. A disorder characterized by hypotrichosis and the appearance of recurrent skin vesicle formation. Affected individuals show sparse and fragile hair on scalp, as well as absent eyebrows and eyelashes. Vesicles filled with thin, watery fluid are observed on the scalp and skin of most of the boby. Mucosal vesicles are absent. -
配列類似性
Contains 5 cadherin domains. -
ドメイン
Calcium may be bound by the cadherin-like repeats. -
細胞内局在
Cell membrane. Cell junction > desmosome. - Information by UniProt
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参照データベース
- Entrez Gene: 1825 Human
- Entrez Gene: 13507 Mouse
- Entrez Gene: 307563 Rat
- Omim: 600271 Human
- SwissProt: Q14574 Human
- SwissProt: P55850 Mouse
- Unigene: 41690 Human
- Unigene: 89935 Mouse
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別名
- Cadherin family member 3 antibody
- CDHF3 antibody
- desmocollin 3a/3b antibody
see all
画像
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All lanes : Anti-Desmocollin 3 antibody [EPR7486] (ab126768) at 1/200000 dilution
Lane 1 : Empty vector transfected 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : 293T cells transfected with myc-his tagged full length Desmocollin-1 expression vector, whole cell lysate
Lane 3 : 293T cells transfected with myc-his tagged full length Desmocollin-2 expression vector, whole cell lysate
Lane 4 : 293T cells transfected with myc-his tagged full length Desmocollin-3 expression vector, whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 100 kDa
Observed band size: 100 kDa
Exposure time: 40 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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ab126768 at 1/10 dilution staining Desmocollin 3 in permeabilized A431 cells by intracellular flow cytometry (red) compared with a rabbit IgG negative control (green).
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All lanes : Anti-Desmocollin 3 antibody [EPR7486] (ab126768) at 1/10000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Human skin lysate
Lane 3 : Human espophagus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 100 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab126768 は 3 報の論文で使用されています。
- Wang J et al. The proliferative and anti-apoptosis functions of KGF/KGFR contributes to bronchial epithelial repair in asthma. Pulm Pharmacol Ther 63:101931 (2020). PubMed: 32818548
- Bir F et al. New immunohistochemical markers in the differential diagnosisof nonsmall cell lung carcinoma. Turk J Med Sci 46:1854-1861 (2016). PubMed: 28081338
- Passantino L et al. Sodium metavanadate exhibits carcinogenic tendencies in vitro in immortalized human bronchial epithelial cells. Metallomics 5:1357-67 (2013). WB ; Human . PubMed: 23963610