Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA/PBS as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (smooth muscle cell line)
Specification
smooth muscle cell line
Permeabilization
Yes - Triton X-100 0.1%
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA/PBS
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 594 goat anti-rabbit IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor 594 + Gold 1.4nm
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor 594 + Gold 1.4nm
Dilution
1/100
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投稿 Mar 31 2015