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  1. Link

    desmin-antibody-de-u-10-cytoskeleton-marker-ab6322.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class III Desmin
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Anti-Desmin 抗体 [DE-U-10] - Cytoskeleton Marker (ab6322)

  • Datasheet
  • SDS
Reviews (5)Q&A (8)References (38)

Product price, shipping and contact information

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申し訳ございませんが、現在表示できません。

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)

Key features and details

  • Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker
    Desmin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: ICC/IF
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Lizard, Snake
  • 免疫原

    Full length native protein (purified) corresponding to Pig Desmin.

  • ポジティブ・コントロール

    • IHC-P: Human skeletal muscle, Mouse cardiac muscle and rat colon tissue.
  • 特記事項

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    DE-U-10
  • ミエローマ

    unknown
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    unknown
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Desmin
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

アプリケーション

Our Abpromise guarantee covers the use of ab6322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for ICC/IF.
  • ターゲット情報

    • 機能

      Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
    • 関連疾患

      Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
      Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
    • 配列類似性

      Belongs to the intermediate filament family.
    • 細胞内局在

      Cytoplasm.
    • Target information above from: UniProt accession P17661 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 280765 Cow
      • Entrez Gene: 1674 Human
      • Entrez Gene: 13346 Mouse
      • Entrez Gene: 64362 Rat
      • Omim: 125660 Human
      • SwissProt: P02542 Chicken
      • SwissProt: O62654 Cow
      • SwissProt: P17661 Human
      • SwissProt: P31001 Mouse
      • SwissProt: P48675 Rat
      • Unigene: 594952 Human
      • Unigene: 6712 Mouse
      • Unigene: 39196 Rat
      see all
    • 別名

      • CMD1I antibody
      • CSM1 antibody
      • CSM2 antibody
      • DES antibody
      • DESM_HUMAN antibody
      • Desmin antibody
      • FLJ12025 antibody
      • FLJ39719 antibody
      • FLJ41013 antibody
      • FLJ41793 antibody
      • Intermediate filament protein antibody
      • OTTHUMP00000064865 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.

      Image shows cytomplasmic staining on mouse cardiac muscle.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.

      Image shows cytomplasmic staining on smooth muscle of rat colon.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)

      IHC image of Desmin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6322, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    プロトコール

    • Immunofluorescence

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (38)

    ab6322 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6322 は 38 報の論文で使用されています。

    • Dye DE  et al. Interaction Between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System. Methods Mol Biol 1889:185-212 (2019). PubMed: 30367415
    • Estañ MC  et al. Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy. Nat Commun 10:797 (2019). PubMed: 30770808
    • Yan D  et al. Neuropeptides Substance P and Calcitonin Gene Related Peptide Accelerate the Development and Fibrogenesis of Endometriosis. Sci Rep 9:2698 (2019). PubMed: 30804432
    • Sato K  et al. Distribution of label-retaining cells and their properties in the vocal fold mucosa. Laryngoscope Investig Otolaryngol 4:76-82 (2019). PubMed: 30828622
    • Powell PC  et al. Chymase uptake by cardiomyocytes results in myosin degradation in cardiac volume overload. Heliyon 5:e01397 (2019). PubMed: 30997426
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 13 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (muscle)
    Permeabilization
    Yes - PBS triton0.5%
    Specification
    muscle
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 14 2016

    Western blot abreview for Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Tissue lysate - whole (skeletal muscle)
    Specification
    skeletal muscle
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Rafael Montes Nieto

    Verified customer

    投稿 Oct 31 2014

    Immunocytochemistry abreview for Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Blocking step
    FBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample
    Human Cultured Cells (immortalized myoblasts)
    Specification
    immortalized myoblasts
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 05 2014

    Question

    IHC-P mouse brain (2 different tumor types)
    no staining
    AR: pH 6 and 10
    Ab: 1/100, 1/200 (5 ug/ml) - prediluted in the morning, used in afternoon
    incubated for 1 h and o/n

    2nd: AP, ABC - works well otherwise

    other primaries work well

    Read More

    Abcam community

    Verified customer

    Asked on Feb 04 2013

    Answer

    I have issued a free of charge replacement with a new lot.

    Read More

    Abcam Scientific Support

    Answered on Feb 04 2013

    Question

    I have tried this anti-desmin antibody for IHC-Fr in mouse brain tissue, perfused with PFA, post-fixed in PFA for 1h and cryoprotected in PB+sucrose. I stained with different protocols and concentrations (permeabilization with trinton x 0.1-0.3% in 10% goat serum for 1h and concentrations ranging from 1:50 to 1:1500). I used different non-abcam gt anti-mouse secondary antibodies. I obtained staining that is not seen in the controls without primary antibody but it is not the expected for desmin. It is staining nucleus of some cells and this is supposed to be a cytoplasmic stain (intermediate filaments).

    I was aiming to stain blood vessels and pericytes with this antibody. I have NG2 antibody that co-stains pericytes and I see those regularly in my staining. This was the first time I used a desmin specific antibody.

    I am attaching a photograph of hyppocampus (below), corpus callosum (medium) and deep layers of cortex (superior). It is labeling the nucleus of pyramidal cells in the hippocampus, not really expected. I am confident because there are pericytes in the brain.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 20 2012

    Answer

    Thank you for sending your protocol, which appears to be correct, and the image file. I agree that the antibody is staining something that is unlikely to be desmin. Are you interested in trying a different antibody against desmin? I see that ab15200 has been published in an article that used it to identify pericytes:

    Schadler KL et al. Delta-like ligand 4 plays a critical role in pericyte/vascular smooth muscle cell formation during vasculogenesis and tumor vessel expansion in Ewing's sarcoma. Clin Cancer Res 16:848-56 (2010). PubMed: 20103680

    Here is the link to the PubMed abstract:

    http://www.ncbi.nlm.nih.gov/pubmed/20103680?dopt=Abstract

    and a link to the antibody datasheet:

    https://www.abcam.com/Desmin-antibody-ab15200.html

    I will be happy to send this as a free-of-charge replacement for ab6322, or a refund if you prefer. Please let me know how you would like to proceed.

    Read More

    Abcam Scientific Support

    Answered on Nov 20 2012

    Western blot abreview for Anti-Desmin antibody [DE-U-10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (skeletal muscle)
    Loading amount
    30 µg
    Specification
    skeletal muscle
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 14 2012

    Question

    Dear Madame or Sir, I would like to ask for a quotation for the following antibodies, please: anti-desmin antibody ab6322 anti-desmin antibody ab15200 And I would like to seek your advices on choosing between these two antibodies. In  specific, I am going to test on human hemaetopoitic progenitor cells. Do you happen to have some information on which one of them will give a more specific staining in this case? (Immunogen for ab15200 polyclonal is synthetic peptide from human, while that for ab6322 monoclonal is pig full length native protein). Any information will be very appreciated. Thank you very much for your attention and best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 06 2011

    Answer

    Thank you for your enquiry whichi has been forwarded to the scientific support team. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications and species listed on the datasheets. Therefore, any Desmin antibody tested in the species and application you are using should be suitable and guaranteed for your experiments. I am sorry to confirm these antibodies have not specifically been tested in human hemaetopoitic progenitor cells. However, I am pleased to let you know that both ab15200 and ab6322 have been tested on human samples and would be covered by our guarantee for use on samples from this species. I can recommend to check the datasheet to view a list of the tested applications to ensure that the application you are using is also tested and guaranteed. The price of products can be found at the top of the datasheet, please ensure to select Singapore from the country selection at the top right hand corner of the page to see the correct price. ab15200 tested in: Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Price USD $365.00 Click here (or use the following: https://www.abcam.com/index.html?datasheet=15200). ab6322 tested in: ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB Price USD $365.00 Click here (or use the following: https://www.abcam.com/index.html?datasheet=6322). Freight/Packing: USD $60.00 to Singapore Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet). I hope this will be helpful to you. Should you have any further questions please do not hesitate to ask.

    Read More

    Abcam Scientific Support

    Answered on Oct 06 2011

    Question

    Would like to test these antibodies in rhesus monkey.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 21 2011

    Answer

    Thanks for your call today. Please see below for your testing discount codes and instructions for use. Let me know if you have any questions! DISCOUNT CODES: (1) ab15463: *** (2) ab6322: *** Expiration date: January 22, 2012 I am very pleased to hear you would like to accept our offer and test these antibodies in rhesus monkey. Each code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody in rhesus samples and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. The codes will be active once the Abreviews have been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreviews and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Sep 21 2011

    Question

    I would like to know if it is possible to get this antibody conjugated with FitC and what the cost would be.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 30 2010

    Answer

    Unfortunately, we do not sell this antibody or any other Desmin antibody in conjugated version. I do not have the information at the moment if and when a conjugated version will be added to the catalogue.

    Read More

    Abcam Scientific Support

    Answered on Nov 30 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-Desmin antibody [DE-U-10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rabbit Tissue sections (Esophagus)
    Specification
    Esophagus
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
    Read More

    Dr. Vyacheslav Ogay

    Verified customer

    投稿 May 15 2007

    1-10 of 13 Abreviews or Q&A

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    For licensing inquiries, please contact partnerships@abcam.com

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