Key features and details
- Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品名Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker
Desmin 一次抗体 製品一覧
製品の詳細Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker
アプリケーション適用あり: IHC-P, IHC-Frmore details
種交差性交差種: Mouse, Rat, Human
交差が予測される動物種: Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Lizard, Snake
Full length native protein (purified) corresponding to Pig Desmin.
- This antibody gave a positive result in IHC in the following FFPE tissue: Human Skeletal muscle.
This antibody clone is manufactured by Abcam.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
精製度Protein G purified
Our Abpromise guarantee covers the use of ab6322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
機能Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
関連疾患Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
配列類似性Belongs to the intermediate filament family.
- Information by UniProt
- CMD1I antibody
- CSM1 antibody
- CSM2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.
Image shows cytomplasmic staining on mouse cardiac muscle.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.
Image shows cytomplasmic staining on smooth muscle of rat colon.
Immunohistochemical analysis of Human colorectal tissue, staining Desmin (green) with ab6322.
Tissue was fixed with ice cold acetone for 5 minutes and blocked with 10% goat serum for 30 minutes. Samples were incubated with primary antibody (1/30) overnight at 4°C and an AlexaFluor®488®-conjugated anti-mouse IgG (1/500) was used as the secondary antibody.
ab6322 at 1/100 staining rabbit oesophagus tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum before incuabion with the antibody for 1 hour. An Alexa-Fluor ® 594 conjugated goat anti-mouse IgG1 was used as the secondary.
IHC image of Desmin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6322, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ab6322 は 38 報の論文で使用されています。
- Dye DE et al. Interaction Between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System. Methods Mol Biol 1889:185-212 (2019). PubMed: 30367415
- Estañ MC et al. Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy. Nat Commun 10:797 (2019). PubMed: 30770808
- Yan D et al. Neuropeptides Substance P and Calcitonin Gene Related Peptide Accelerate the Development and Fibrogenesis of Endometriosis. Sci Rep 9:2698 (2019). PubMed: 30804432
- Sato K et al. Distribution of label-retaining cells and their properties in the vocal fold mucosa. Laryngoscope Investig Otolaryngol 4:76-82 (2019). PubMed: 30828622
- Powell PC et al. Chymase uptake by cardiomyocytes results in myosin degradation in cardiac volume overload. Heliyon 5:e01397 (2019). PubMed: 30997426