Anti-Desmin 抗体 [DE-U-10] - Cytoskeleton Marker (ab6322)

Reviews (5)Q&A (8)References (46)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)

Key features and details

  • Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker
    Desmin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [DE-U-10] to Desmin - Cytoskeleton Marker

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Rabbit, Goat, Chicken, Hamster, Cow, Cat, Lizard, Snake

  • 免疫原

    Full length native protein (purified) corresponding to Pig Desmin.

  • ポジティブ･コントロール

    • IHC-P: Human skeletal muscle, Mouse cardiac muscle and rat colon tissue.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6322の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.

  • 関連疾患

    Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
    Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.

  • 配列類似性

    Belongs to the intermediate filament family.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CMD1I antibody
    • CSM1 antibody
    • CSM2 antibody
    • DES antibody
    • DESM_HUMAN antibody
    • Desmin antibody
    • FLJ12025 antibody
    • FLJ39719 antibody
    • FLJ41013 antibody
    • FLJ41793 antibody
    • Intermediate filament protein antibody
    • OTTHUMP00000064865 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.

    Image shows cytomplasmic staining on mouse cardiac muscle.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.

    Image shows cytomplasmic staining on smooth muscle of rat colon.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322)

    IHC image of Desmin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6322, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

参考文献 (46)

ab6322 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6322 は 46 報の論文で使用されています。

  • Deligne C  et al. Development of a human in vitro blood-brain tumor barrier model of diffuse intrinsic pontine glioma to better understand the chemoresistance. Fluids Barriers CNS 17:37 (2020). PubMed: 32487241
  • Du M  et al. Targeting NFATc4 attenuates non-alcoholic steatohepatitis in mice. J Hepatol 73:1333-1346 (2020). PubMed: 32717288
  • Wang F  et al. BMSC-derived leptin and IGFBP2 promote erlotinib resistance in lung adenocarcinoma cells through IGF-1R activation in hypoxic environment. Cancer Biol Ther 21:61-71 (2020). PubMed: 31559898
  • Ali II  et al. Oxytocin induces intracellular Ca2+ release in cardiac fibroblasts from neonatal rats. Cell Calcium 84:102099 (2019). PubMed: 31614270
  • Nederveen JP  et al. Consistent expression pattern of myogenic regulatory factors in whole muscle and isolated human muscle satellite cells after eccentric contractions in humans. J Appl Physiol (1985) 127:1419-1426 (2019). PubMed: 31513447
View all Publications for this product

レビューと Q&A

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1-10 of 13 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker

 Inconclusive
Abreviews
This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (muscle)
Permeabilization
Yes - PBS triton0.5%
Specification
muscle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 14 2016

Western blot abreview for Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Tissue lysate - whole (skeletal muscle)
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

DR. Rafael Montes Nieto

Verified customer

投稿 Oct 31 2014

Immunocytochemistry abreview for Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Blocking step
FBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Human Cultured Cells (immortalized myoblasts)
Specification
immortalized myoblasts
Permeabilization
No
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Sep 05 2014

Western blot abreview for Anti-Desmin antibody [DE-U-10]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Loading amount
30 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jun 14 2012

Immunohistochemistry (Frozen sections) abreview for Anti-Desmin antibody [DE-U-10]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (Esophagus)
Specification
Esophagus
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
Read More

Dr. Vyacheslav Ogay

Verified customer

投稿 May 15 2007

Question

IHC-P mouse brain (2 different tumor types)
no staining
AR: pH 6 and 10
Ab: 1/100, 1/200 (5 ug/ml) - prediluted in the morning, used in afternoon
incubated for 1 h and o/n

2nd: AP, ABC - works well otherwise

other primaries work well

Read More
Answer

I have issued a free of charge replacement with a new lot.

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Question

I have tried this anti-desmin antibody for IHC-Fr in mouse brain tissue, perfused with PFA, post-fixed in PFA for 1h and cryoprotected in PB+sucrose. I stained with different protocols and concentrations (permeabilization with trinton x 0.1-0.3% in 10% goat serum for 1h and concentrations ranging from 1:50 to 1:1500). I used different non-abcam gt anti-mouse secondary antibodies. I obtained staining that is not seen in the controls without primary antibody but it is not the expected for desmin. It is staining nucleus of some cells and this is supposed to be a cytoplasmic stain (intermediate filaments).

I was aiming to stain blood vessels and pericytes with this antibody. I have NG2 antibody that co-stains pericytes and I see those regularly in my staining. This was the first time I used a desmin specific antibody.

I am attaching a photograph of hyppocampus (below), corpus callosum (medium) and deep layers of cortex (superior). It is labeling the nucleus of pyramidal cells in the hippocampus, not really expected. I am confident because there are pericytes in the brain.

Read More
Answer

Thank you for sending your protocol, which appears to be correct, and the image file. I agree that the antibody is staining something that is unlikely to be desmin. Are you interested in trying a different antibody against desmin? I see that ab15200 has been published in an article that used it to identify pericytes:

Schadler KL et al. Delta-like ligand 4 plays a critical role in pericyte/vascular smooth muscle cell formation during vasculogenesis and tumor vessel expansion in Ewing's sarcoma. Clin Cancer Res 16:848-56 (2010). PubMed: 20103680

Here is the link to the PubMed abstract:

http://www.ncbi.nlm.nih.gov/pubmed/20103680?dopt=Abstract

and a link to the antibody datasheet:

https://www.abcam.com/Desmin-antibody-ab15200.html

I will be happy to send this as a free-of-charge replacement for ab6322, or a refund if you prefer. Please let me know how you would like to proceed.

Read More

Question

Dear Madame or Sir, I would like to ask for a quotation for the following antibodies, please: anti-desmin antibody ab6322 anti-desmin antibody ab15200 And I would like to seek your advices on choosing between these two antibodies. In  specific, I am going to test on human hemaetopoitic progenitor cells. Do you happen to have some information on which one of them will give a more specific staining in this case? (Immunogen for ab15200 polyclonal is synthetic peptide from human, while that for ab6322 monoclonal is pig full length native protein). Any information will be very appreciated. Thank you very much for your attention and best regards,

Read More
Answer

Thank you for your enquiry whichi has been forwarded to the scientific support team. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications and species listed on the datasheets. Therefore, any Desmin antibody tested in the species and application you are using should be suitable and guaranteed for your experiments. I am sorry to confirm these antibodies have not specifically been tested in human hemaetopoitic progenitor cells. However, I am pleased to let you know that both ab15200 and ab6322 have been tested on human samples and would be covered by our guarantee for use on samples from this species. I can recommend to check the datasheet to view a list of the tested applications to ensure that the application you are using is also tested and guaranteed. The price of products can be found at the top of the datasheet, please ensure to select Singapore from the country selection at the top right hand corner of the page to see the correct price. ab15200 tested in: Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Price USD $365.00 Click here (or use the following: https://www.abcam.com/Desmin-antibody-Cytoskeleton-Marker-ab15200.html). ab6322 tested in: ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB Price USD $365.00 Click here (or use the following: https://www.abcam.com/Desmin-antibody-DE-U-10-Cytoskeleton-Marker-ab6322.html). Freight/Packing: USD $60.00 to Singapore Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done. If you wish to be more certain, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet). I hope this will be helpful to you. Should you have any further questions please do not hesitate to ask.

Read More

Question

Would like to test these antibodies in rhesus monkey.

Read More
Answer

Thanks for your call today. Please see below for your testing discount codes and instructions for use. Let me know if you have any questions! DISCOUNT CODES: (1) ab15463: *** (2) ab6322: *** Expiration date: January 22, 2012 I am very pleased to hear you would like to accept our offer and test these antibodies in rhesus monkey. Each code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for each antibody in rhesus samples and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. The codes will be active once the Abreviews have been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreviews and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question

I would like to know if it is possible to get this antibody conjugated with FitC and what the cost would be.

Read More
Answer

Unfortunately, we do not sell this antibody or any other Desmin antibody in conjugated version. I do not have the information at the moment if and when a conjugated version will be added to the catalogue.

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1-10 of 13 Abreviews or Q&A

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