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    desmin-antibody-d33-ab8470.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class III Desmin
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Anti-Desmin 抗体 [D33] (ab8470)

  • Datasheet
  • SDS
Reviews (6)Q&A (4)References (27)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [D33] (ab8470)

    Key features and details

    • Mouse monoclonal [D33] to Desmin
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    こちらの製品もご検討ください

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    関連製品

    製品の概要

    • 製品名

      Anti-Desmin antibody [D33]
      Desmin 一次抗体 製品一覧
    • 製品の詳細

      Mouse monoclonal [D33] to Desmin
    • 由来種

      Mouse
    • 特異性

      This antibody is highly reactive with desmin. On immunoblots only the 56 kD desmin band is stained. Non reactive with GFAP, Keratin, Vimentin and Neurofilament.
    • アプリケーション

      適用あり: IHC-Pmore details
    • 種交差性

      交差種: Human
    • 免疫原

      Tissue, cells or virus. Immunogen derived from human Leiomyoma.
      Database link: P17661

    • 特記事項

      Sufficient antibody for 100-500 tests


      This antibody stains muscle cells in human tissues

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • バッファー

      pH: 7.3
      Preservative: 0.1% Sodium azide
      Constituent: 1% BSA
    • Concentration information loading...
    • 精製度

      Tissue culture supernatant
    • 一次抗体 備考

      This antibody stains muscle cells in human tissues
    • ポリ/モノ

      モノクローナル
    • クローン名

      D33
    • アイソタイプ

      IgG1
    • 研究分野

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Intermediate Filaments
      • Class III
      • Desmin
      • Stem Cells
      • Mesenchymal Stem Cells
      • Myogenesis
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • Other
      • Cardiovascular
      • Heart
      • Cardiac arrhythmias

    関連製品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab8470の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P (6)
    1/100.
    特記事項
    IHC-P
    1/100.

    ターゲット情報

    • 機能

      Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
    • 関連疾患

      Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
      Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
      Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
    • 配列類似性

      Belongs to the intermediate filament family.
    • 細胞内局在

      Cytoplasm.
    • Target information above from: UniProt accession P17661 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 1674 Human
      • Omim: 125660 Human
      • SwissProt: P17661 Human
      • Unigene: 594952 Human
      • 別名

        • CMD1I antibody
        • CSM1 antibody
        • CSM2 antibody
        • DES antibody
        • DESM_HUMAN antibody
        • Desmin antibody
        • FLJ12025 antibody
        • FLJ39719 antibody
        • FLJ41013 antibody
        • FLJ41793 antibody
        • Intermediate filament protein antibody
        • OTTHUMP00000064865 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [D33] (ab8470)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [D33] (ab8470)
        IHC image of ab8470 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8470, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      プロトコール

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (27)

      ab8470 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab8470 は 27 報の論文で使用されています。

      • Diesinger T  et al. A New CYP2E1 Inhibitor, 12-Imidazolyl-1-dodecanol, Represents a Potential Treatment for Hepatocellular Carcinoma. Can J Gastroenterol Hepatol 2021:8854432 (2021). PubMed: 33604316
      • Tan JL  et al. Restoration of the healing microenvironment in diabetic wounds with matrix-binding IL-1 receptor antagonist. Commun Biol 4:422 (2021). PubMed: 33772102
      • Günther HS  et al. GFAP and desmin expression in lymphatic tissues leads to difficulties in distinguishing between glial and stromal cells. Sci Rep 11:13322 (2021). PubMed: 34172765
      • Post Y  et al. Snake Venom Gland Organoids. Cell 180:233-247.e21 (2020). PubMed: 31978343
      • Hu G  et al. Chronic exercise provides renal-protective effects with upregulation of fatty acid oxidation in the kidney of high fructose-fed rats. Am J Physiol Renal Physiol 318:F826-F834 (2020). PubMed: 32036700
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 10 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [D33]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Hamster Tissue sections (Muscle)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
      Permeabilization
      No
      Specification
      Muscle
      Blocking step
      BSA + Milk + BFS as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 27°C
      Fixative
      Formaldehyde
      Read More

      Dr. Marcelo Pelajo Machado

      Verified customer

      投稿 May 25 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [D33]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Chinchilla lanigera Tissue sections (Uterus, Myometrium, smooth muscle cells)
      Antigen retrieval step
      Heat mediated
      Permeabilization
      No
      Specification
      Uterus, Myometrium, smooth muscle cells
      Blocking step
      Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 24°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Mar 24 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [D33]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Sheep Tissue sections (Lung)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: EnVision FLEX Target Retrieval Solution
      Permeabilization
      No
      Specification
      Lung
      Blocking step
      (agent) for 10 minute(s) · Concentration: 3% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Jan 16 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [D33]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rabbit Tissue sections (Muscle)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Cytrate pH6
      Permeabilization
      No
      Specification
      Muscle
      Blocking step
      Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Nov 09 2016

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [D33]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Dog Tissue sections (Myocytes of blood vessel)
      Antigen retrieval step
      Heat mediated
      Permeabilization
      No
      Specification
      Myocytes of blood vessel
      Blocking step
      Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Antoine Veron

      Verified customer

      投稿 Jul 12 2016

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody [D33]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Cat Tissue sections (Muscle of leg)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Cytrate pH6
      Permeabilization
      No
      Specification
      Muscle of leg
      Blocking step
      Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Antoine Veron

      Verified customer

      投稿 Jul 12 2016

      Question

      I had a question regarding this product: On the data sheet, this product states to be a monoclonal. What is the clone number?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 12 2006

      Answer

      Thank you for your enquiry. Ab8470 is clone D33. I have added this information to our online datasheet. Please contact us again if you have any additional questions.

      Read More

      Abcam Scientific Support

      Answered on Jan 13 2006

      Question

      I have just received the feedback from our customer. She send a protocoll and the answers to your questions: Staining Protokoll: 1. dewax 30 minutes, 100%Alkohol, 96 % Alkohol, 80% Alkohol, 70% Alkohol: 6 minutes 2. dest. water: 5 min. 3. Mikrowave: 800 watt, 2 x 5 min. in citrate buffer for antigen retrieval: Cooling for 20 min. 4. Using Dako Pen, 2 x 5 min TBS Buffer 5. 3 % H2O2 in dest. Water for 20 min. 6. 2 x 5 min. TBST buffer 7. Normal Serum from rabbit: 10 % in BSA(1%)/TBS: 90 min. in sandwich box 8: prim. Antibody 1:50 in PBS: overnight at 5°C in sandwich box ( ca. 18 hours) 9: 2 x 5 min. TBST buffer 10. Sek. Antibody: 1:100 in BSA/TBS: 90 min. at Roomtemperature in sandwich box. 11. 2x 5 min. TBST buffer 12. Avidin-Biotin Komplex in BSA/TBS: 30 min. in sandwich box 13. 2 x 5 min. TBS buffer 14. DAB (Dako) : 10 min. in sandwich box. 15. dest. water: 5 min. 16. Hämatoxylin (Mayer) : 2-3 min., water Question 1: No staining in skelatal muscle from neck or temple. Question 2: Human muscle from heart, colon: positiv staining, but not in every case Human muscle from neck or temple: no staining Question 3: 4% Formaldehyd buffered Question 4 and 5: Microwave: s. Protokoll Question 6: Mouse monoclonal to Desmin igG1 (ab 8470) testet dilutions: 1:50, 1:100, 1:200 tested incubation times: overnight at 5°C, 2 hours at 37°C Question 7: Polyclonal Rabbit Anti Mouse iG , biotinylated Rabbit F(ab')2 reacts with all mouse IgG subclasses and mouse IgM, and it will probably also react with other mouse immunoglobulins via their light chains. I dont think, that the problems coming from the secondary antibody. I become a positiv staining with materail from colon and heart. tested dilutions: 1:50, 1:100, 1:200 tested incubation: 1 hour at 37°C, 90 min. RT, 120 min. RT Question 8: a very low background staining Question 10: Negativ controlls: Tissue from Dermis, Tissue without prim. Antibody Positiv controlls: Tissue from colon Question 11: IHC in skelatal muscle: 8 times: everytime negativ IHC in smooth muscle: 2 times: positiv, with best results in some tissues from heart and colon. I choose cases from 2002 to 2004. Different regions from heart and colon. But not everywhere I will become a positiv result. But from 90 % and that everytime. I changed the dilution, the solvent, the time, the temperature, detection method: ABC or LSAB II Hopefully this will help to clarify the case. If I can be of any further assistence, please let me know.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 22 2004

      Answer

      Thank you for your patience. The antibody should work very well in skeletal muscle, unless it is myopathic. With skeletal myopathy lack of demin occurs. We recommend as positive controls skeleton muscle, rhabdomyosarcomas and leiomyoma cells. We would also advise to use pretreatment: Heat Induced Epitope Retrieval (HIER) techniques.

      Read More

      Abcam Scientific Support

      Answered on Jun 29 2004

      Question

      Have you tested this antibody for reactivity on other species such as mouse ? If is not the case, it is possible to buy a small quantity to test it. Thanks Peggy ADIN.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 30 2002

      Answer

      This antibody has not been tested in any other species except human. Unfortunately, it is not possible to purchase a smaller quantity for testing purposes.

      Read More

      Abcam Scientific Support

      Answered on Apr 30 2002

      Question

      Have you tested this antibody for reactivity on other species such as rat? If not based on your experience with other Ab of this type would it be reasonable to test? Thanks, John Williams

      Read More

      Abcam community

      Verified customer

      Asked on Dec 26 2001

      Answer

      Cross reactivity with rat has not yet been tested. Unfortunately we do not think that this antibody will cross react with rat.

      Read More

      Danielle Miller

      Abcam Scientific Support

      Answered on Jan 15 2002

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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