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    desmin-antibody-cytoskeleton-marker-ab15200.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class III Desmin
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Anti-Desmin 抗体 - Cytoskeleton Marker (ab15200)

  • Datasheet
  • SDS
Reviews (26)Q&A (6)References (183)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

Key features and details

  • Rabbit polyclonal to Desmin - Cytoskeleton Marker
  • Suitable for: ICC, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Desmin antibody [Y66] - Cytoskeleton Marker (ab32362)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Desmin antibody - Cytoskeleton Marker
    Desmin 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Desmin - Cytoskeleton Marker
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Desmin aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P17661

  • ポジティブ・コントロール

    • ICC: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), C2C12, RMS13, and A431 cells. IHC-P: Human skeletal muscle, human and rat cardiac muscle.
  • 特記事項

    The negative control used to test this Ab was: skin (squamous epithelial cells) was used as negative control tissue.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Desmin
    • Stem Cells
    • Mesenchymal Stem Cells
    • Myogenesis
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Signal Transduction
    • Antibodies
    • desmin

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab15200の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC (2)
Use a concentration of 0.1 - 1 µg/ml.
IHC-P (13)
1/200.
特記事項
ICC
Use a concentration of 0.1 - 1 µg/ml.
IHC-P
1/200.

ターゲット情報

  • 機能

    Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.
  • 関連疾患

    Defects in DES are the cause of myopathy myofibrillar desmin-related (MFM-DES) [MIM:601419]; also known as desmin-related myopathy (DRM). A neuromuscular disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by myofibrillar destruction with intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells.
    Defects in DES are the cause of cardiomyopathy dilated type 1I (CMD1I) [MIM:604765]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in DES are the cause of neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome) [MIM:181400]. Kaeser syndrome is an autosomal dominant disorder with a peculiar scapuloperoneal distribution of weakness and atrophy. A large clinical variability is observed ranging from scapuloperoneal, limb grindle and distal phenotypes with variable cardiac or respiratory involvement. Facial weakness, dysphagia and gynaecomastia are frequent additional symptoms. Affected men seemingly bear a higher risk of sudden, cardiac death as compared to affected women. Histological and immunohistochemical examination of muscle biopsy specimens reveal a wide spectrum of findings ranging from near normal or unspecific pathology to typical, myofibrillar changes with accumulation of desmin.
  • 配列類似性

    Belongs to the intermediate filament family.
  • 細胞内局在

    Cytoplasm.
  • Target information above from: UniProt accession P17661 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 1674 Human
    • Entrez Gene: 13346 Mouse
    • Entrez Gene: 64362 Rat
    • Omim: 125660 Human
    • SwissProt: P17661 Human
    • SwissProt: P31001 Mouse
    • SwissProt: P48675 Rat
    • Unigene: 594952 Human
    • Unigene: 6712 Mouse
    • Unigene: 39196 Rat
    see all
  • 別名

    • CMD1I antibody
    • CSM1 antibody
    • CSM2 antibody
    • DES antibody
    • DESM_HUMAN antibody
    • Desmin antibody
    • FLJ12025 antibody
    • FLJ39719 antibody
    • FLJ41013 antibody
    • FLJ41793 antibody
    • Intermediate filament protein antibody
    • OTTHUMP00000064865 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunohistochemical analysis of paraffin-embedded on human skeletal muscle labeling Desmin with ab15200 at 1/200 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.

  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunofluorescence staining of Desmin using ab15200 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.


    The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab15200 at 0.1 µg/mL and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).


    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.


    The antibody ab15200 gave comparable results using MeOH fixation (100%, 5 min).

  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunocytochemistry/Immunofluorescence analysis of C2C12 (mouse muscle) cells labelling Desmin with ab15200 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)This image is courtesy of an Abreview submitted by Dr Mal Niladri

    ab15200 staining rat differentiated skeletal muscle cells by ICC/IF.

    Cultured differentiated skeletal muscle cells were harvested from Lewis rats, 2% paraformaldehyde treated for 15 min to fix the cells, and then permealized with Triton-X100 (0.1%) for 10 min. The cells were then incubated with ab15200 at 1/200 overnight at 4°C. The image was taken with a confocal laser scanning microscope and shows desmin expressing skeletal myocytes (green-cytoplasmic localization). Note that desmin forms short thickened filamentous structures in the cell cytoplasm, along with prominent spot-like cytoplasmic aggregates that are composed of densely packed filaments.

    See Abreview

  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunocytochemistry/Immunofluorescence analysis of RMS13 (human rhabdomyosarcoma) cells labelling Desmin with ab15200 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells (negative cell line) showing weak staining of Desmin with ab15200 at 1/500. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunocytochemistry - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)This image is courtesy of an anonymous abreview.

    ab15200 staining Desmin in rat Smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in PSS for 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300 in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An MFP 555-conjugated donkey polyclonal to rabbit IgG was used as secondary antibody at 1/400 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunohistochemical analysis of paraffin-embedded on human cardiac muscle labeling Desmin with ab15200 at 1/200 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)

    Immunohistochemical analysis of paraffin-embedded on rat cardiac muscle labeling Desmin with ab15200 at 1/200 dilution followed by goat anti-rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)This image is courtesy of an anonymous abreview.

    ab15200 staining Desmin in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1/800 in blocking buffer) for 16 hours at 4°C. An undiluted HRP-conjugated Horse anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Ab15200 positively staining desmin in formaldehyde fixed mouse heart tissue (1/200). Ab15200 was used in conjunction with goat anti rabbit (biotin).

    The reviewer reported no difference in the quality of staining obtained when enzymatic epitope unmasking was preformed.

    This image is an edited version of an image submitted courtesy of an Abreview by Khaled Chatila on 11 October 2005. For further information relating to the protocol please refer to the Abreview.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody - Cytoskeleton Marker (ab15200)This image is courtesy of an anonymous abreview.

    Immunohistochemical analysis of PFA-fixed paraffin-embedded murine nephric tissue, labelling Desmin with ab15200 at a dilution of 1/250 incubated for 18 hours at 4°C. Heat mediated antigen retrival was with citrate buffer. Blocking was with Dako serum-free protein block at 100% incubated for 1 hour at 23°C. Secondary was a donkey anti-rabbit polyclonal HRP conjugate at 1/200.

    See Abreview

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (183)

ab15200 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab15200 は 183 報の論文で使用されています。

  • Suzuki K  et al. A new method of primary engineering of esophagus using orthotopic in-body tissue architecture. J Pediatr Surg 56:1186-1191 (2021). PubMed: 33845983
  • Hein S  et al. Impaired in vitro growth response of plasma-treated cardiomyocytes predicts poor outcome in patients with transthyretin amyloidosis. Clin Res Cardiol 110:579-590 (2021). PubMed: 33481097
  • Chen H  et al. A structure-supporting, self-healing, and high permeating hydrogel bioink for establishment of diverse homogeneous tissue-like constructs. Bioact Mater 6:3580-3595 (2021). PubMed: 33869899
  • Locci E  et al. Metabolomics improves the histopathological diagnosis of asphyxial deaths: an animal proof-of-concept model. Sci Rep 11:10102 (2021). PubMed: 33980966
  • Uzunalli G  et al. Structural disruption of the blood-brain barrier in repetitive primary blast injury. Fluids Barriers CNS 18:2 (2021). PubMed: 33413513
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 32 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Pig Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 100x Citrate Buffer pH 6.0(ab64236)
Permeabilization
Yes - 0.05% tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jun 30 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Mar 16 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 25 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% tween 20
Specification
liver
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 13 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% tween 20
Specification
Colon
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 13 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% tween 20
Specification
heart
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 13 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% tween 20
Specification
heart
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 13 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Desmin antibody - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% tween 20
Specification
kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

投稿 Feb 13 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Desmin antibody - Cytoskeleton Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney H9 organoids)
Permeabilization
Yes - 0.05% Tween 20
Specification
kidney H9 organoids
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Feb 13 2020

Question

Could you suggest secondary antibody for antiDesmin antibody ab15200.

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Abcam community

Verified customer

Asked on Apr 30 2012

Answer

Thank you for your inquiry.

We have a number of antibodies in our catalogue that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com).

Please click the secondary antibody tab at the top of the home page. A advance search will be generated which can be further refined using the filters on the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

As we discussed on phone; please check the following link for free prism ladder;

https://www.abcam.com/index.html?pageconfig=resource&rid=14968

I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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Abcam Scientific Support

Answered on Apr 30 2012

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