遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-DEPDC5 抗体 [EPR20497-23] - BSA and Azide free (ab272399)

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Western blot - Anti-DEPDC5 antibody [EPR20497-23] - BSA and Azide free (ab272399)
  • Anti-DEPDC5 antibody [EPR20497-23] - BSA and Azide free (ab272399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20497-23] to DEPDC5 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse

製品の概要

  • 製品名

    Anti-DEPDC5 antibody [EPR20497-23] - BSA and Azide free
    DEPDC5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20497-23] to DEPDC5 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Wild-type mouse E14 brain lysate.

  • 特記事項

    ab272399 is the carrier-free version of ab213181. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
WB
Mouse
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 181 kDa (predicted molecular weight: 181 kDa).
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 181 kDa (predicted molecular weight: 181 kDa).
  • 追加情報
    Is unsuitable for IHC-P or IP.

    • ターゲット情報

    画像

    • Western blot - Anti-DEPDC5 antibody [EPR20497-23] - BSA and Azide free (ab272399)
      Western blot - Anti-DEPDC5 antibody [EPR20497-23] - BSA and Azide free (ab272399)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The wild-type and DEPDC5 knockout mouse E14 brain tissue lysates were kindly provided by an anonymous collaborator.

      ab213181 was shown to specifically react with DEPDC5 in wild-type mouse E14 brain tissue as signal was lost in DEPDC5 knockout tissue. Wild-type and DEPDC5 knockout samples were subjected to SDS-PAGE. ab213181 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100, 000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique. 

      Exposure time: 26 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab213181).

    • Anti-DEPDC5 antibody [EPR20497-23] - BSA and Azide free (ab272399)
      Anti-DEPDC5 antibody [EPR20497-23] - BSA and Azide free (ab272399)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272399 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272399 は論文での使用が確認できていません。

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