Anti-DEP1 抗体 (ab202958)
Key features and details
- Rabbit polyclonal to DEP1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DEP1 antibody
DEP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DEP1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human DEP1 aa 850-950 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q12913 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab202958の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods. |
特記事項 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use at 1/50 - 1/200 with fluorescent detection methods. |
ターゲット情報
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機能
Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling. -
組織特異性
Expressed in the promyelocytic cell line HL60, the granulocyte-macrophage colony-stimulating factor-dependent leukemic cell line F-36P, and the interleukin-3 and erythropoietin-dependent leukemic cell line F-36E. Expressed predominantly in epithelial cells and lymphocytes. Enhanced expression at high cell density. -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.
Contains 9 fibronectin type-III domains.
Contains 1 tyrosine-protein phosphatase domain. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Cell membrane. Cell projection > ruffle membrane. Cell junction. After T cell stimulation, it is temporarily excluded from immunological synapses. - Information by UniProt
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参照データベース
- Entrez Gene: 5795 Human
- Omim: 600925 Human
- SwissProt: Q12913 Human
- Unigene: 318547 Human
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別名
- CD 148 antibody
- CD148 antibody
- CD148 antigen antibody
see all
画像
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human lung carcinoma tissue sections labeling DEP1 with ab202958 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human colon carcinoma tissue sections labeling DEP1 with ab202958 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab202958 は 1 報の論文で使用されています。
- Tsagianni A et al. Combined Systemic Disruption of MET and Epidermal Growth Factor Receptor Signaling Causes Liver Failure in Normal Mice. Am J Pathol 188:2223-2235 (2018). PubMed: 30031724