Anti-DDX56/DDX21 抗体 (ab97648)
Key features and details
- Rabbit polyclonal to DDX56/DDX21
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DDX56/DDX21 antibody
DDX56/DDX21 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DDX56/DDX21 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Recombinant fragment corresponding to Human DDX56/DDX21 aa 251-403. (AAH01235)
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ポジティブ･コントロール
- A549, HeLa, HepG2 and HCT116 cell lines; MCF7 xenograft ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97648の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/500.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 62 kDa.
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| ICC/IF |
1/100 - 1/500.
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| 特記事項 |
|---|
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IHC-P
1/100 - 1/500. |
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WB
1/500 - 1/3000. Predicted molecular weight: 62 kDa. |
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ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity. -
組織特異性
Detected in heart, brain, liver, pancreas, placenta and lung. -
配列類似性
Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 508728 Cow
- Entrez Gene: 54606 Human
- Entrez Gene: 52513 Mouse
- Entrez Gene: 289780 Rat
- Omim: 608023 Human
- SwissProt: Q3SZ40 Cow
- SwissProt: Q9NY93 Human
- SwissProt: Q9D0R4 Mouse
see all -
別名
- 61 kd nucleolar helicase antibody
- ATP-dependent 61 kDa nucleolar RNA helicase antibody
- DDX21 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-DDX56/DDX21 antibody (ab97648)
Paraformaldehyde-fixed HeLa cells stained for DDX56/DDX21 using ab97648 at 1/500 dilution in ICC/IF (green). Alpha tubulin filaments are detected with an ant-tubulin antibody (red).
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Western blot - Anti-DDX56/DDX21 antibody (ab97648)Anti-DDX56/DDX21 antibody (ab97648) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 62 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-DDX56/DDX21 antibody (ab97648)ab97648, at a 1/200 dilution, staining DDX56/DDX21 in paraformaldehyde fixed HeLa cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56/DDX21 antibody (ab97648)ab97648, at a 1/500 dilution, staining DDX56/DDX21 in formalin fixed, paraffin embedded MCF7 xenograft by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab97648 は 1 報の論文で使用されています。
- Li D et al. DDX56 inhibits type I interferon by disrupting assembly of IRF3-IPO5 to inhibit IRF3 nucleus import. J Cell Sci 133:N/A (2019). PubMed: 31340999