Anti-DDX5 抗体 [EPR7240] - BSA and Azide free (ab248227)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7240] to DDX5 - BSA and Azide free
- Suitable for: IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-DDX5 antibody [EPR7240] - BSA and Azide free
DDX5 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7240] to DDX5 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
ab248227 is the carrier-free version of ab128928.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7240 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Conjugation kits
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248227の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa).
|
|
ICC/IF |
Use at an assay dependent concentration.
|
特記事項 |
---|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa). |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
-
機能
RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing. -
配列類似性
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
翻訳後修飾
Arg-502 is dimethylated, probably to asymmetric dimethylarginine. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
-
参照データベース
- Entrez Gene: 1655 Human
- Entrez Gene: 13207 Mouse
- Entrez Gene: 287765 Rat
- Omim: 180630 Human
- SwissProt: P17844 Human
- SwissProt: Q61656 Mouse
- Unigene: 279806 Human
- Unigene: 220038 Mouse
-
別名
- ATP dependent RNA helicase DDX5 antibody
- DDX 5 antibody
- Ddx5 antibody
see all
画像
-
This data was developed using ab128928, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: DDX5 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab128928 observed at 69 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab128928 was shown to specifically react with DDX5 in wild-type cells as signal was lost in DDX5 knockout cells. Wild-type and DDX5 knockout samples were subjected to SDS-PAGE. ab128928 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-DDX5 antibody [EPR7240] (ab128928) at 1/1000 dilution
Lane 1 : HT-29 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 69 kDaThis data was developed using ab128928, the same antibody clone in a different buffer formulation.
-
This data was developed using ab128928, the same antibody clone in a different buffer formulation.ab128928 at 1/100 dilution staining DDX5 in paraffin-embedded Human breast carcinoma tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab248227 は論文での使用が確認できていません。