Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (anal cancer)
Specification
anal cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Blocking step
Sequential pre-diluted peroxidase (10 min) and protein (10 min) blocks. as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
40 minute(s) · Temperature: 20°C · Diluent: antibody diluent
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
could be used more diluted - would recommend range of 1/1000 – 1/5000
we do not have stage/grade info for the cancer.
Blocks were pre-diluted from Dako peroxidase block (S2001) followed by serum-free protein block (X0909) - nethier contains detergent.
we do not have stage/grade info for the cancer.
Blocks were pre-diluted from Dako peroxidase block (S2001) followed by serum-free protein block (X0909) - nethier contains detergent.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
投稿 Jul 17 2008