Anti-DDX46 抗体 (ab72083)
Key features and details
- Rabbit polyclonal to DDX46
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-DDX46 antibody -
製品の詳細
Rabbit polyclonal to DDX46 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
免疫原
Synthetic peptide corresponding to Human DDX46.
Database link: Q7L014 -
ポジティブ・コントロール
- IHC-P: Human breast carcinoma. WB: HeLa, 293T, NIH/3T3 whole cell lysate. IP: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72083の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 117 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 117 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Plays an essential role in splicing, either prior to, or during splicing A complex formation. -
細胞内局在
Nucleus speckle. Nucleus, Cajal body. -
参照データベース
- Entrez Gene: 9879 Human
- Entrez Gene: 212880 Mouse
- Entrez Gene: 100626833 Pig
- Entrez Gene: 245957 Rat
- Entrez Gene: 711026 Rhesus monkey
- SwissProt: Q7L014 Human
- SwissProt: Q569Z5 Mouse
- SwissProt: Q62780 Rat
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別名
- DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 antibody
- DEAD box protein 46 antibody
- Probable ATP dependent RNA helicase DDX46 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling DDX46 with ab72083 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-DDX46 antibody (ab72083) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH/3T3 cells at 50 µg
Predicted band size: 117 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa, 77 kDa. We are unsure as to the identity of these extra bands. -
Analysis of Human DDX46 by Immunoprecipitation in whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells using ab72083 at 3 µg/mg lysate (lane 1) or a control rabbit IgG (lane 2). ab72083 was used for WB detection at 1 µg/ml. Detection: chemioluminescence, exposure time 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab72083 は 2 報の論文で使用されています。
- Lin Q et al. DDX46 silencing inhibits cell proliferation by activating apoptosis and autophagy in cutaneous squamous cell carcinoma. Mol Med Rep 22:4236-4242 (2020). PubMed: 33000271
- Zheng Q et al. The RNA helicase DDX46 inhibits innate immunity by entrapping m6A-demethylated antiviral transcripts in the nucleus. Nat Immunol 18:1094-1103 (2017). WB . PubMed: 28846086