Anti-DDX3 抗体 (ab95979)
Key features and details
- Rabbit polyclonal to DDX3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-DDX3 antibody
DDX3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DDX3 -
由来種
Rabbit -
特異性
The immunogen sequence is derived from DDX3Y, the DDX3X homologous sequence is 95% identical so should also be detected byt his antibody.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment, corresponding to a sequence within amino acids 70-316 of Human DDX3Y.
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ポジティブ・コントロール
- 293T, A431, H1299, HeLa, HepG2, Molt4, and Raji cells and whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95979の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 73 kDa.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 73 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. -
配列類似性
Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
細胞内局在
Nucleus speckle. Cytoplasm. Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1-dependent manner. - Information by UniProt
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参照データベース
- Entrez Gene: 1654 Human
- Entrez Gene: 13205 Mouse
- Entrez Gene: 317335 Rat
- Omim: 300160 Human
- SwissProt: O00571 Human
- SwissProt: Q62167 Mouse
- Unigene: 380774 Human
- Unigene: 289662 Mouse
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別名
- ATP dependent RNA helicase DDX3X antibody
- ATP-dependent RNA helicase DDX3X antibody
- CAP Rf antibody
see all
画像
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All lanes : Anti-DDX3 antibody (ab95979) at 1/10000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa -
ab95979 at 1/200 dilution staining DDX3 in HeLa cells by Immunofluorescence, Paraformaldehyde-fixed. Lower image is merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab95979 は論文での使用が確認できていません。