Anti-DDDDK tag (Binds to FLAG® tag sequence) 抗体 (HRP) (ab2493)
Key features and details
- Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence) (HRP)
- Suitable for: ELISA, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
製品の概要
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製品名
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (HRP)
DDDDK tag (Binds to FLAG® tag sequence) 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence) (HRP) -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence).
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特記事項
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
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アプリケーション
Our Abpromise guarantee covers the use of ab2493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | 1/5000 - 1/20000. | |
| WB | 1/1000 - 1/10000. |
ターゲット情報
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関連性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
別名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
参考文献 (2)
ab2493 は 2 報の論文で使用されています。
- Kling E et al. Convergent evolution of integration site selection upstream of tRNA genes by yeast and amoeba retrotransposons. Nucleic Acids Res 46:7250-7260 (2018). PubMed: 29945249
- Belashov IA et al. Structure of HIV TAR in complex with a Lab-Evolved RRM provides insight into duplex RNA recognition and synthesis of a constrained peptide that impairs transcription. Nucleic Acids Res 46:6401-6415 (2018). PubMed: 29961805
