Key features and details
- Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence) (FITC)
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
製品名Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (FITC)
DDDDK tag (Binds to FLAG® tag sequence) 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence) (FITC)
標識FITC. Ex: 493nm, Em: 528nm
アプリケーション適用あり: WBmore details
種交差性交差種: Species independent
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence). Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
The molar F/P ratio is 4.1.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Store at +4°C.
バッファーPreservative: 0.1% Sodium azide
Constituent: 0.2% BSA
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab2492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Optimal working dilutions should be determined for your particular assay conditions.
関連性This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence).
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
ab2492 は 3 報の論文で使用されています。
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- Guo Q et al. TCN, an AKT inhibitor, exhibits potent antitumor activity and enhances radiosensitivity in hypoxic esophageal squamous cell carcinoma in vitro and in vivo. Oncol Lett 13:949-954 (2017). PubMed: 28356983