Anti-DDDDK tag (Binds to FLAG® tag sequence) 抗体 [FG4R] (ab125243)
Key features and details
- Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB, ELISA, Dot blot, IP, In situ hybridization
- Reacts with: Species independent
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R]
DDDDK tag (Binds to FLAG® tag sequence) 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence) -
由来種
Mouse -
特異性
Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged (also known as the FLAG® tag sequence) fusion proteins.
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アプリケーション
適用あり: WB, ELISA, Dot blot, IP, In situ hybridizationmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.
Sequence:DYKDDDDK
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ポジティブ・コントロール
- HEK293 cells transfected with DYKDDDDK (also known as the FLAG® tag sequence).
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特記事項
If stored at 4°C please add 0.1% sodium azide.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
ab125243 was purified by protein A affinity chromatography from TCS -
ポリ/モノ
モノクローナル -
クローン名
FG4R -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125243の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/3000.
(with ECL) |
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ELISA |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
|
|
In situ hybridization |
1/500 - 1/2000.
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特記事項 |
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WB
1/1000 - 1/3000. (with ECL) |
ELISA
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
In situ hybridization
1/500 - 1/2000. |
ターゲット情報
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関連性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
別名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab125243 は 28 報の論文で使用されています。
- Yang G et al. The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling. Front Microbiol 13:911479 (2022). PubMed: 35770165
- Wang D et al. SIRPα maintains macrophage homeostasis by interacting with PTK2B kinase in Mycobacterium tuberculosis infection and through autophagy and necroptosis. EBioMedicine 85:104278 (2022). PubMed: 36202053
- Wang Y et al. Engineering antiviral immune-like systems for autonomous virus detection and inhibition in mice. Nat Commun 13:7629 (2022). PubMed: 36494373
- Guo M et al. Proliferation of Highly Cytotoxic Human Natural Killer Cells by OX40L Armed NK-92 With Secretory Neoleukin-2/15 for Cancer Immunotherapy. Front Oncol 11:632540 (2021). PubMed: 33937033
- Fan C et al. Teleost-Specific MxG, a Traitor in the Mx Family, Negatively Regulates Antiviral Responses by Targeting IPS-1 for Proteasomal Degradation and STING for Lysosomal Degradation. J Immunol 207:281-295 (2021). PubMed: 34135063