Anti-DDDDK tag (Binds to FLAG® tag sequence) 抗体 (ab1257)
製品の概要
-
製品名
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody
DDDDK tag (Binds to FLAG® tag sequence) 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to DDDDK tag (Binds to FLAG® tag sequence) -
由来種
Goat -
アプリケーション
適用あり: ELISA, WB, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:xxxDDDDK
-
特記事項
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | 1/100 - 1/500. | |
| WB | 1/1000 - 1/30000. | |
| ICC/IF | 1/200 - 1/2000. |
ターゲット情報
-
関連性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
別名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1257)Human microvascular endothelial cells expressing DDDDK-tagged beta-catenin following transient transfection.
Ab1257 used at 1 µg/ml. Detected with FITC labeled rabbit anti-goat IgG (H&L) secondary antibody. -
Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1257)All lanes : Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1257) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate. at 0.2 µg
Lane 2 : E. coli whole cell lysate. at 0.1 µg
Lane 3 : E. coli whole cell lysate. at 0.05 µgWestern Blot analysis of E. coli Whole Cell Lysates, labelled with DDDDK-tagged Protein by ab1257 at 1/25,000.
プロトコール
参考文献
ab1257 は 31 報の論文で使用されています。
- Xu W et al. Endothelial FAM3A positively regulates post-ischaemic angiogenesis. EBioMedicine 43:32-42 (2019). PubMed: 31000420
- Yin G et al. miR-221-3p promotes the cell growth of non-small cell lung cancer by targeting p27. Mol Med Rep 20:604-612 (2019). PubMed: 31180541
- Park HS et al. NS1 Protein of 2009 Pandemic Influenza A Virus Inhibits Porcine NLRP3 Inflammasome-Mediated Interleukin-1 Beta Production by Suppressing ASC Ubiquitination. J Virol 92:N/A (2018). PubMed: 29386291
- Zhang MQ et al. Differential Effects of Autophagy-Related 10 Protein on HCV Replication and Autophagy Flux Are Mediated by Its Cysteine44 and Cysteine135. Front Immunol 9:2176 (2018). PubMed: 30319633
- Liu G et al. Nuclear-resident RIG-I senses viral replication inducing antiviral immunity. Nat Commun 9:3199 (2018). PubMed: 30097581
