Anti-DDDDK tag (Binds to FLAG® tag sequence) 抗体 (ab1170)
製品の概要
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製品名
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody
DDDDK tag (Binds to FLAG® tag sequence) 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to DDDDK tag (Binds to FLAG® tag sequence) -
由来種
Chicken -
アプリケーション
適用あり: ELISA, WB, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.
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特記事項
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were affinity purified using the peptide coupled to a different carrier. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | 1/5000 - 1/20000. | |
| WB | 1/2000 - 1/10000. | |
| ICC/IF | Use at an assay dependent concentration. |
ターゲット情報
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関連性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
別名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170)This image is courtesy of an anonymous Abreviewab1170 staining DDDDK in Mouse primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 1x PBS + 5% Goat serum + 0.1% Triton-X 100) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated Goat anti-chicken IgY polyclonal (1/400) was used as the secondary antibody.
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Western blot - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170)This image is courtesy of an anonymous AbreviewAnti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1170) at 1/5000 dilution
Secondary
Rabbit Anti-Chicken IgY H&L (HRP) (ab6753) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 66 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
プロトコール
参考文献
ab1170 は 2 報の論文で使用されています。
- Lim J et al. Nuclear Proximity of Mtr4 to RNA Exosome Restricts DNA Mutational Asymmetry. Cell 169:523-537.e15 (2017). PubMed: 28431250
- Hutson MR et al. Temperature-activated ion channels in neural crest cells confer maternal fever-associated birth defects. Sci Signal 10:N/A (2017). PubMed: 29018170
