Anti-DDDDK tag (Binds to FLAG® tag sequence) 抗体 (ab1162)
Key features and details
- Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: ICC, WB, ELISA
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody
DDDDK tag (Binds to FLAG® tag sequence) 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to DDDDK tag (Binds to FLAG® tag sequence) -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WB, ELISAmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence). Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.
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特記事項
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1162の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC | (1) |
Use at an assay dependent concentration.
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WB | (9) |
Use a concentration of 0.2 - 1 µg/ml.
Antibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope" R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope. |
ELISA |
Use a concentration of 1 - 5 µg/ml.
As coating antibody in sELISA. |
特記事項 |
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ICC
Use at an assay dependent concentration. |
WB
Use a concentration of 0.2 - 1 µg/ml. Antibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope" R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope. |
ELISA
Use a concentration of 1 - 5 µg/ml. As coating antibody in sELISA. |
ターゲット情報
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関連性
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
別名
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
画像
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Immunofluorescent analysis of A293 cells labeling FLAG®-VPS35 with ab1162 at 10 μg/ml. The nuclear counter stain is DAPI (blue).
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Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)This image is courtesy of an anonymous Abreviewab1162 staining DDDDK tag in hTERT-RPE1 by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Saponin and blocked with 0.02% BSA for 20 minutes at 18°C. Samples were incubated with primary antibody (1/100) for 45 minutes at 18°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody.
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Immunocytochemistry - Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody (ab1162)Image from Kapoor N et al., J Biol Chem. 2011 Apr 22;286(16):14073-9. Epub 2011 Jan 4. Fig 1A.; doi: 10.1074/jbc.M110.185298; April 22, 2011 The Journal of Biological Chemistry, 286, 14073-14079.
ab1162 staining DDDDK tag (pink) in neonatal rat cardiomyocytes transduced with Ad-TBX18/FLAG®-IRES-GFP by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and incubated with primary antibody. An Alexa Fluor®-conjugated anti-rabbit IgG was used as the secondary antibody. Nuclei were stained with DAPI (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (89)
ab1162 は 89 報の論文で使用されています。
- Ma YY et al. IL28A protein homotetramer structure is required for autolysosomal degradation of HCV-NS5A in vitro. Cell Death Dis 11:200 (2020). PubMed: 32205851
- Binder JL et al. Optical induction of autophagy via Transcription factor EB (TFEB) reduces pathological tau in neurons. PLoS One 15:e0230026 (2020). PubMed: 32208437
- Showalter AE et al. Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis. Sci Rep 10:798 (2020). PubMed: 31964905
- Jones RS et al. Monocarboxylate Transporter 6-Mediated Interactions with Prostaglandin F2a: In Vitro and In Vivo Evidence Utilizing a Knockout Mouse Model. Pharmaceutics 12:N/A (2020). PubMed: 32110957
- Tian C et al. MCTS1 Directly Binds to TWF1 and Synergistically Modulate Cyclin D1 and C-Myc Translation in Luminal A/B Breast Cancer Cells. Onco Targets Ther 13:5353-5361 (2020). PubMed: 32606753