Application
Western blot
Sample
Escherichia coli Purified protein (Mach1cell)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
0.5 µg
Specification
Mach1cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBST+5%Milk
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 66 kDa
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投稿 Sep 09 2009